Rabbit anti-Human KLHL25 Polyclonal Antibody | anti-KLHL25 antibody

KLHL25, NT (KLHL25, ENC2, Ectoderm-neural cortex protein 2, Kelch-like protein 25) (FITC)

Cat. Num. AAA6314164

• Gene Names: KLHL25; ENC2; ENC-2
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: KLHL25; Polyclonal Antibody; NT (KLHL25; ENC2; Ectoderm-neural cortex protein 2; Kelch-like protein 25) (FITC); Kelch-like protein 25; anti-KLHL25 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-KLHL25 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: KLHL25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 61-91 amino acids from the N-terminal region of human KLHL25.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(KLHL25 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the KLHL25 antibody detected the KLHL25 protein (arrow).)

Product Categories/Family for anti-KLHL25 antibody

Antibodies; Kelch-like Proteins

References

Lunetta, K.L., et al. BMC Med. Genet. 8 SUPPL 1, S13 (2007): Simpson, J.C., et al. EMBO Rep. 1(3):287-292(2000).

NCBI and Uniprot Product Information

• NCBI GI #: 21362105
• NCBI GeneID: 64410
• NCBI Accession #: NP_071925.2
• NCBI GenBank Nucleotide #: NM_022480.3
• UniProt Accession #: Q9H0H3; B2RDH2; B3KRT7
• Molecular Weight: 65,923 Da
• NCBI Official Full Name: kelch-like protein 25
• NCBI Official Synonym Full Names: kelch-like family member 25
• NCBI Official Symbol: KLHL25
• NCBI Official Synonym Symbols: ENC2; ENC-2
• NCBI Protein Information: kelch-like protein 25; kelch-like 25; ectodermal-neural cortex 2; BTB/POZ KELCH domain protein; ectoderm-neural cortex protein 2
• UniProt Protein Name: Kelch-like protein 25
• Protein Family: Kelch-like protein
• UniProt Gene Name: KLHL25
• UniProt Synonym Gene Names: ENC2; ENC-2
• UniProt Entry Name: KLH25_HUMAN

Uniprot Description

KLHL25: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex required for translational homeostasis. The BCR(KLHL25) ubiquitin ligase complex acts by mediating ubiquitination of hypophosphorylated EIF4EBP1 (4E-BP1): ubiquitination and subsequent degradation of hypophosphorylated EIF4EBP1 (4E-BP1) probably serves as a homeostatic mechanism to maintain translation and prevent eIF4E inhibition when eIF4E levels are low. The BCR(KLHL25) complex does not target EIF4EBP1 (4E-BP1) when it is hyperphosphorylated or associated with eIF4E. Component of the BCR(KLHL25) E3 ubiquitin ligase complex, at least composed of CUL3, KLHL25 and RBX1. Interacts with EIF4EBP1 (when hypophosphorylated)

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 15q25.3

Cellular Component: cytoplasm

Biological Process: protein ubiquitination during ubiquitin-dependent protein catabolic process; protein ubiquitination; regulation of translational initiation