Mouse XK-related protein 5 ELISA Kit | XKR5 elisa kit
Mouse XK-related protein 5 (XKR5) ELISA Kit
Gene Names
Xkr5; XRG5; 5430438H03Rik
Reactivity
Mouse
Synonyms
XK-related protein 5; Mouse XK-related protein 5 (XKR5) ELISA Kit; XKR5 elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse XKR5. No significant cross-reactivity or interference between Mouse XKR5 and analogues was observed.
Sequence Length
666
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for XKR5 elisa kit
XKR5 belongs to the XK family. XK is a protein found on human red blood cells and other tissues which is responsible for the Kx antigen which helps determine a person's blood type. The Kx antigen plays a role in matching blood for blood transfusions. Absence of XK protein leads to McLeod syndrome, a multi-system disorder characterized by hemolytic anemia, myopathy, acanthocytosis, and chorea.
XK is located on the X chromosome and absence of the XK protein is an X-linked disease. XK is a membrane transport protein of unknown action.The XK locus is inactivated by lyonization. The precursor missing in McLeod's red cells is called Kx. The X-linked locus determining this substance is called XK.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate XKR5 in samples. An antibody specific for XKR5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyXKR5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for XKR5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of XKR5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
XK is located on the X chromosome and absence of the XK protein is an X-linked disease. XK is a membrane transport protein of unknown action.The XK locus is inactivated by lyonization. The precursor missing in McLeod's red cells is called Kx. The X-linked locus determining this substance is called XK.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate XKR5 in samples. An antibody specific for XKR5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyXKR5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for XKR5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of XKR5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
74,634 Da
NCBI Official Full Name
XK-related protein 5 isoform a
NCBI Official Synonym Full Names
X-linked Kx blood group related 5
NCBI Official Symbol
Xkr5
NCBI Official Synonym Symbols
XRG5; 5430438H03Rik
NCBI Protein Information
XK-related protein 5
UniProt Protein Name
XK-related protein 5
Protein Family
UniProt Gene Name
Xkr5
UniProt Synonym Gene Names
Xrg5
Uniprot Description
XKR5: Belongs to the XK family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 8|8 A1.3
Cellular Component: integral component of membrane; membrane
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Product Notes
The Mouse XKR5 xkr5 (Catalog #AAA280392) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA280392 ELISA Kit recognizes Mouse XKR5. It is sometimes possible for the material contained within the vial of "XK-related protein 5, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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