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SDS-Page

WNT7A recombinant protein

WNT7A, 32-349aa, Human, His tag, E Coli (Denatured)

Applications
SDS-Page
Purity
> 85% by SDS-PAGE
Synonyms
WNT7A; 32-349aa; Human; His tag; E Coli (Denatured); Protein Wnt-7a precursor; Wingless-type MMTV integration site family; member7A; WNT7A recombinant protein
Ordering
For Research Use Only!
Host
E Coli
Purity/Purification
> 85% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol, 0.4M urea
Concentration
1mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MGSLGASIIC NKIPGLAPRQ RAICQSRPDA IIVIGEGSQM GLDECQFQFR NGRWNCSALG ERTVFGKELK VGSREAAFTY AIIAAGVAHA ITAACTQGNL SDCGCDKEKQ GQYHRDEGWK WGGCSADIRY GIGFAKVFVD AREIKQNART LMNLHNNEAG RKILEENMKL ECKCHGVSGS CTTKTCWTTL PQFRELGYVL KDKYNEAVHV EPVRASRNKR PTFLKIKKPL SYRKPMDTDL VYIEKSPNYC EEDPVTGSVG TQGRACNKTA PQASGCDLMC CGRGYNTHQY ARVWQCNCKF HWCCYVKCNT CSERTEMYTC K
Sequence Length
349
Applicable Applications for WNT7A recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

SDS-Page

SDS-Page
Related Product Information for WNT7A recombinant protein
WNT7A is a member of the WNT gene family, which consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is involved in the development of the anterior-posterior axis in the female reproductive tract, and also plays a critical role in uterine smooth muscle pattering and maintenance of adult uterine function. Mutations in this gene are associated with Fuhrmann and Al-Awadi/Raas-Rothschild/Schinzel phocomelia syndromes. Recombinant human WNT7A protein, fused to His-tag at N-terminus, was expressed in E Coli.
Product Categories/Family for WNT7A recombinant protein
References
Bui T.D., Lako M.. et al. (1997). Gene 189:25-29

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
38.0 kDa (341aa)
NCBI Official Full Name
protein Wnt-7a
NCBI Official Synonym Full Names
Wnt family member 7A
NCBI Official Symbol
WNT7A
NCBI Protein Information
protein Wnt-7a
UniProt Protein Name
Protein Wnt-7a
UniProt Gene Name
WNT7A

NCBI Description

This gene is a member of the WNT gene family, which consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is involved in the development of the anterior-posterior axis in the female reproductive tract, and also plays a critical role in uterine smooth muscle pattering and maintenance of adult uterine function. Mutations in this gene are associated with Fuhrmann and Al-Awadi/Raas-Rothschild/Schinzel phocomelia syndromes. [provided by RefSeq, Jul 2008]

Uniprot Description

Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. Signaling by Wnt-7a allows sexually dimorphic development of the mullerian ducts ().

Research Articles on WNT7A

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Product Notes

The WNT7A wnt7a (Catalog #AAA205253) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's WNT7A can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the WNT7A wnt7a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MGSLGASIIC NKIPGLAPRQ RAICQSRPDA IIVIGEGSQM GLDECQFQFR NGRWNCSALG ERTVFGKELK VGSREAAFTY AIIAAGVAHA ITAACTQGNL SDCGCDKEKQ GQYHRDEGWK WGGCSADIRY GIGFAKVFVD AREIKQNART LMNLHNNEAG RKILEENMKL ECKCHGVSGS CTTKTCWTTL PQFRELGYVL KDKYNEAVHV EPVRASRNKR PTFLKIKKPL SYRKPMDTDL VYIEKSPNYC EEDPVTGSVG TQGRACNKTA PQASGCDLMC CGRGYNTHQY ARVWQCNCKF HWCCYVKCNT CSERTEMYTC K. It is sometimes possible for the material contained within the vial of "WNT7A, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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