Human Protein Wnt-3a ELISA Kit | WNT3A elisa kit
Human Protein Wnt-3a (WNT3A) ELISA Kit
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate WNT3A in Human serum, plasma. An antibody specific for WNT3A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any WNT3A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WNT3A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WNT3A bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI Description
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 96% amino acid identity to mouse Wnt3A protein, and 84% to human WNT3 protein, another WNT gene product. This gene is clustered with WNT14 gene, another family member, in chromosome 1q42 region. [provided by RefSeq, Jul 2008]
Uniprot Description
WNT3A: Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube. Interacts with PORCN. Interacts with APCDD1 and WLS. Component of the Wnt-Fzd-LRP5-LRP6 signaling complex that contains a WNT protein, a FZD protein and LRP5 or LRP6. Interacts directly in the complex with LRP6. Moderately expressed in placenta and at low levels in adult lung, spleen, and prostate. Belongs to the Wnt family.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 1q42.13
Cellular Component: cell surface; early endosome membrane; endoplasmic reticulum lumen; extracellular region; extracellular space; Golgi lumen; plasma membrane; proteinaceous extracellular matrix
Molecular Function: frizzled binding; frizzled-2 binding; protein binding; protein domain specific binding; receptor binding; receptor ligand activity; transcription coactivator activity
Biological Process: axon guidance; cell proliferation in forebrain; cell proliferation in midbrain; COP9 signalosome assembly; dorsal/ventral neural tube patterning; extracellular matrix organization; heart looping; hemopoiesis; hippocampus development; in utero embryonic development; inner ear morphogenesis; mammary gland development; negative regulation of axon extension involved in axon guidance; negative regulation of fat cell differentiation; negative regulation of neurogenesis; neuron differentiation; osteoblast differentiation; palate development; paraxial mesodermal cell fate commitment; positive regulation of B cell proliferation; positive regulation of cell proliferation; positive regulation of collateral sprouting in the absence of injury; positive regulation of cysteine-type endopeptidase activity involved in apoptotic process; positive regulation of cytokine production; positive regulation of mesodermal cell fate specification; positive regulation of peptidyl-serine phosphorylation; positive regulation of protein binding; positive regulation of protein phosphorylation; positive regulation of receptor internalization; positive regulation of skeletal muscle tissue development; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-templated; spinal cord association neuron differentiation; Wnt receptor signaling pathway through beta-catenin; Wnt signaling pathway; Wnt signaling pathway involved in forebrain neuroblast division
Research Articles on WNT3A
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Product Notes
The Human WNT3A wnt3a (Catalog #AAA281216) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA281216 ELISA Kit recognizes Human WNT3A. It is sometimes possible for the material contained within the vial of "Protein Wnt-3a, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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