Mouse WD repeat domain phosphoinositide-interacting protein 2 ELISA Kit | WIPI2 elisa kit
Mouse WD repeat domain phosphoinositide-interacting protein 2 (WIPI2) ELISA Kit
Gene Names
Wipi2; 1110018O08Rik; 2510001I10Rik
Reactivity
Mouse
Synonyms
WD repeat domain phosphoinositide-interacting protein 2; Mouse WD repeat domain phosphoinositide-interacting protein 2 (WIPI2) ELISA Kit; WIPI2 elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse WIPI2. No significant cross-reactivity or interference between Mouse WIPI2 and analogues was observed.
Sequence Length
454
Samples
Serum, plasma and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
62.5 pg/mL - 4000 pg/mL
Sensitivity
31.25 pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for WIPI2 elisa kit
Intended Uses: For the quantitative detection of Mouse WD repeat domain phosphoinositide- interacting protein 2 (WIPI2) concentration in serum, plasma and other biological fluids.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate WIPI2 in Mouse serum, plasma. An antibody specific for WIPI2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any WIPI2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WIPI2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WIPI2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate WIPI2 in Mouse serum, plasma. An antibody specific for WIPI2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any WIPI2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WIPI2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WIPI2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
48,477 Da
NCBI Official Full Name
WD repeat domain phosphoinositide-interacting protein 2
NCBI Official Synonym Full Names
WD repeat domain, phosphoinositide interacting 2
NCBI Official Symbol
Wipi2
NCBI Official Synonym Symbols
1110018O08Rik; 2510001I10Rik
NCBI Protein Information
WD repeat domain phosphoinositide-interacting protein 2
UniProt Protein Name
WD repeat domain phosphoinositide-interacting protein 2
Protein Family
UniProt Gene Name
Wipi2
UniProt Synonym Gene Names
WIPI-2
Uniprot Description
WIPI2: Probable early component of the autophagy machinery being involved in formation of preautophagosomal structures and their maturation into mature phagosomes in response to PtdIns3P. May bind PtdIns3P. Interacts with TECPR1. Ubiquitously expressed. Highly expressed in heart, skeletal muscle and pancreas. Expression is down-regulated in pancreatic and in kidney tumors. Belongs to the WD repeat SVP1 family. 6 isoforms of the human protein are produced by alternative splicing.
Protein type: Autophagy
Chromosomal Location of Human Ortholog: 5|5 G2
Cellular Component: autophagic vacuole; cytosol; extrinsic component of membrane; membrane; pre-autophagosomal structure membrane; protein complex
Molecular Function: phosphatidylinositol 3-phosphate binding; phosphatidylinositol-5-phosphate binding; protein binding
Biological Process: autophagosome assembly; autophagy; mitochondrion degradation; protein lipidation
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Product Notes
The Mouse WIPI2 wipi2 (Catalog #AAA282413) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA282413 ELISA Kit recognizes Mouse WIPI2. It is sometimes possible for the material contained within the vial of "WD repeat domain phosphoinositide-interacting protein 2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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