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Western Blot (WB) ((1ug/ml) staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Goat CPT1C Polyclonal Antibody | anti-CPT1C antibody

Goat anti-CPT1C Antibody

Gene Names
CPT1C; CATL1; CPT1P; CPTIC; SPG73; CPT1-B; CPTI-B
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
CPT1C; Polyclonal Antibody; Goat anti-CPT1C Antibody; carnitine palmitoyltransferase 1C; CATL1; CPT1P; CPTIC; FLJ23809; CPT1-like pseudogene; carnitine acyltransferase-like protein 1; carnitine palmitoyltransferase I related C; CPT1C antibody; carnitine palmitoyltransferase 1C antibody; CATL1 antibody; CPT1P antibody; CPTIC antibody; FLJ23809 antibody; CPT1-like pseudogene antibody; carnitine acyltransferase-like protein 1 antibody; carnitine palmitoyltransferase I related C antibody; anti-CPT1C antibody
Ordering
For Research Use Only!
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Applicable Applications for anti-CPT1C antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 8000.
Western Blot: Approx. 100kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 91. 0kDa according to NP_689572.1). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Testis. Recommended concentration: 3.75ug/ml
Immunogen
Peptide with sequence C-KSSTKTDSHRLGQH, from the internal region of the protein sequence according to NP_689572.1; NP_001129524.1.
Note
This antibody is expected to recognise both reported isoforms (NP_689572.1; NP_001129524.1). Reported variants represent identical protein (NP_001186681.1; NP_001186682.1; NP_689572.1).
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((1ug/ml) staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB) ((1ug/ml) staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
carnitine O-palmitoyltransferase 1, brain isoform isoform 2
NCBI Official Synonym Full Names
carnitine palmitoyltransferase 1C
NCBI Official Symbol
CPT1C
NCBI Official Synonym Symbols
CATL1; CPT1P; CPTIC; SPG73; CPT1-B; CPTI-B
NCBI Protein Information
carnitine O-palmitoyltransferase 1, brain isoform
UniProt Protein Name
Carnitine O-palmitoyltransferase 1, brain isoform
UniProt Gene Name
CPT1C
UniProt Synonym Gene Names
CATL1; CPT1-B; CPTI-B
UniProt Entry Name
CPT1C_HUMAN

NCBI Description

This gene encodes a member of the carnitine/choline acetyltransferase family. The encoded protein regulates the beta-oxidation and transport of long-chain fatty acids into mitochondria, and may play a role in the regulation of feeding behavior and whole-body energy homeostasis. Alternatively spliced transcript variants encoding multiple protein isoforms have been observed for this gene. [provided by RefSeq, Dec 2010]

Uniprot Description

CPT1C: a member of the carnitine/choline acetyltransferase family. The encoded protein regulates the beta-oxidation and transport of long-chain fatty acids into mitochondria, and may play a role in the regulation of feeding behavior and whole-body energy homeostasis. Alternatively spliced transcript variants encoding multiple protein isoforms have been observed for this gene. [provided by RefSeq, Dec 2010]

Protein type: EC 2.3.1.21; Transferase; Lipid Metabolism - fatty acid; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: axon; cell junction; dendrite; endoplasmic reticulum; endoplasmic reticulum membrane; mitochondrial outer membrane; synapse

Molecular Function: carnitine O-palmitoyltransferase activity; protein binding

Biological Process: carnitine metabolic process; fatty acid beta-oxidation

Disease: Spastic Paraplegia 73, Autosomal Dominant

Research Articles on CPT1C

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Product Notes

The CPT1C cpt1c (Catalog #AAA421995) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-CPT1C Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's CPT1C can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 8000. Western Blot: Approx. 100kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 91. 0kDa according to NP_689572.1). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour. IHC: Paraffin embedded Human Testis. Recommended concentration: 3.75ug/ml. Researchers should empirically determine the suitability of the CPT1C cpt1c for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CPT1C, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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