Human WD repeat domain, phosphoinositide interacting 1 ELISA Kit | WIPI1 elisa kit

Human WD repeat domain phosphoinositide-interacting protein 1, WIPI1 ELISA Kit

Cat. Num. AAA9335866

• Gene Names: WIPI1; ATG18; ATG18A; WIPI49
• Reactivity: Human
• Synonyms: WD repeat domain; phosphoinositide interacting 1; Human WD repeat domain phosphoinositide-interacting protein 1; WIPI1 ELISA Kit; Human WD repeat domain phosphoinositide-interacting protein 1 (WIPI1) ELISA kit; ATG18; FLJ10055; WIPI49; WD40 repeat protein Interacting with phosphoInositides of 49kDa; WIPI-1 alpha; WIPI1 elisa kit

• Reactivity: Human

• Samples: Undiluted original Human serum, plasma or tissue homogenates samples
• Assay Type: Quantitative Sandwich
• Detection Range: 0.625ng/ml-20ng/ml
• Sensitivity: 0.1ng/ml
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]. All CV% should be compared by concentration, not compared by OD values.
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for WIPI1 elisa kit

Background/Introduction: This Quantitative Sandwich ELISA kit is for lab reagent/research use only! This kit is intended to be used for determine the level of WIPI1 (hereafter termed "analyte") in undiluted original Human serum, plasma or tissue homogenates samples. For other sample types please contact tech support to determine compatibility with this assay. This kit is not suitable for assaying non-biological sources of substances.

NCBI and Uniprot Product Information

• NCBI GI #: 157388939
• NCBI GeneID: 55062
• NCBI Accession #: NP_060453.3
• NCBI GenBank Nucleotide #: NM_017983.5
• UniProt Accession #: Q5MNZ9; Q8IXM5; Q9NWF8
• Molecular Weight: 48,673 Da
• NCBI Official Full Name: WD repeat domain phosphoinositide-interacting protein 1
• NCBI Official Synonym Full Names: WD repeat domain, phosphoinositide interacting 1
• NCBI Official Symbol: WIPI1
• NCBI Official Synonym Symbols: ATG18; ATG18A; WIPI49
• NCBI Protein Information: WD repeat domain phosphoinositide-interacting protein 1; WIPI 49 kDa; WIPI-1 alpha; atg18 protein homolog; WD40 repeat protein Interacting with phosphoInositides of 49kDa; WD40 repeat protein interacting with phosphoinositides of 49 kDa
• UniProt Protein Name: WD repeat domain phosphoinositide-interacting protein 1
• Protein Family: WD repeat domain phosphoinositide-interacting protein
• UniProt Gene Name: WIPI1
• UniProt Synonym Gene Names: WIPI49; WIPI-1; WIPI 49 kDa
• UniProt Entry Name: WIPI1_HUMAN

NCBI Description

WD40 repeat proteins are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI subfamily of WD40 repeat proteins, such as WIPI1, have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids (Proikas-Cezanne et al., 2004 [PubMed 15602573]).[supplied by OMIM, Mar 2008]

Uniprot Description

ATG18: May play a role in autophagy. May regulate the trafficking of proteins involved in the mannose-6-phosphate receptor (MPR) recycling pathway. Probably interacts with androgen (AR) and the estrogen receptor (ER). Binds PtdIns3P and to a lesser extent, PtdIns3,5P2 and PtdIns5P in vitro. Interaction with PtdIns3P is required for recruitment to membranes. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Autophagy

Chromosomal Location of Human Ortholog: 17q24.2

Cellular Component: Golgi membrane; cytoskeleton; extrinsic to membrane; clathrin-coated vesicle; pre-autophagosomal structure membrane; cytoplasm; endosome membrane; trans-Golgi network; cytosol

Molecular Function: androgen receptor binding; estrogen receptor binding; phosphatidylinositol 3-phosphate binding; receptor binding

Biological Process: unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; unfolded protein response; protein amino acid lipidation; mitochondrion degradation; autophagy; cellular response to nitrogen starvation; vesicle targeting, trans-Golgi to endosome; autophagic vacuole formation