Tight Junction Protein 3 (TJP3) Recombinant Protein | TJP3 recombinant protein

Recombinant Tight Junction Protein 3 (TJP3)

Cat. Num. AAA2086904

• Gene Names: TJP3; ZO3; ZO-3
• Applications: SDS-Page, Western Blot
• Purity: > 90%
• Synonyms: Tight Junction Protein 3 (TJP3); Recombinant Tight Junction Protein 3 (TJP3); ZO-3; Zona Occludens 3; Zonula occludens protein 3; TJP3 recombinant protein

• Host: E Coli
• Purity/Purification: > 90%
• Form/Format: Freeze-dried powder
• Sequence Length: 919

• Applicable Applications for TJP3 recombinant protein: Positive Control, Immunogen, SDS-PAGE, Western Blot (WB)
• Fragment: Met1~Leu210
• Tag: N-terminal His-tag
• Buffer Formulation: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Source Note: Prokaryotic expression
• Organism Species: Human
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Isoelectric Point: 10.2
• Subcellular Location: Cell Junction. Cell Membrane. Membrane. Tight Junction.
• Usage: Reconstitute in PBS or others.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

Product Categories/Family for TJP3 recombinant protein

Recombinant Protein

NCBI and Uniprot Product Information

• NCBI GI #: 389565493
• NCBI GeneID: 27134
• NCBI Accession #: NP_001254489.1
• NCBI GenBank Nucleotide #: NM_001267560.1
• UniProt Accession #: O95049; Q32N01; A6NFP3; B3KR73; B3KXZ0; B4E2W6; F5H2X0; F5H4S9; K7EK22
• Molecular Weight: 26.4kDa
• NCBI Official Full Name: tight junction protein ZO-3 isoform 1
• NCBI Official Synonym Full Names: tight junction protein 3
• NCBI Official Symbol: TJP3
• NCBI Official Synonym Symbols: ZO3; ZO-3
• NCBI Protein Information: tight junction protein ZO-3
• UniProt Protein Name: Tight junction protein ZO-3
• Protein Family: Tight junction protein
• UniProt Gene Name: TJP3
• UniProt Synonym Gene Names: ZO3

NCBI Description

The protein encoded by this gene is a member of the membrane-associated guanylate kinase-like (MAGUK) protein family which is characterized by members having multiple PDZ domains, a single SH3 domain, and a single guanylate kinase-like (GUK)-domain. In addition, members of the zonula occludens protein subfamily have an acidic domain, a basic arginine-rich region, and a proline-rich domain. The protein encoded by this gene plays a role in the linkage between the actin cytoskeleton and tight-junctions and also sequesters cyclin D1 at tight junctions during mitosis. Alternative splicing results in multiple transcript variants encoding distinct isoforms. This gene has a partial pseudogene on chromosome 1. [provided by RefSeq, May 2012]

Uniprot Description

ZO3: Interacts with occludin, claudins and ZO-1. Interacts with INADL. Interacts with UBN1. Interacts with FASLG. Belongs to the MAGUK family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: nucleus; tight junction

Molecular Function: protein binding