HE4 Recombinant Protein | WFDC2 recombinant protein

HE4 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121200

• Purity: >95% as determined by SDS-PAGE
• Synonyms: HE4; HE4 Protein; Human; Recombinant (His Tag); Human WAP5/WFDC2/HE4 Protein (His Tag); dJ461P17.6 Protein; EDDM4 Protein; WAP5 Protein; WAP four-disulfide core domain 2; WFDC2 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Phe124

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Glu 31
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human WFDC2 (NP_006094.3)(Met 1-Phe 124) was expressed, with a C-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for WFDC2 recombinant protein

Background: WAP four-disulfide core domain protein 2, also known as Epididymal secretory protein E4, Major epididymis-specific protein E4, Putative protease inhibitor WAP5, WFDC2 and HE4, is a secreted protein that contains two WAP domains. WFDC2/HE4 is a member of a family of stable 4-disulfide core proteins that are secreted at high levels. It is expressed in a number of normal tissues, including male reproductive system, regions of the respiratory tract and nasopharynx. It is highly expressed in a number of tumors cells lines, such ovarian, colon, breast, lung and renal cells lines. Initially described as being exclusively transcribed in the epididymis. WFDC2 may be a component of the innate immune defences of the lung, nasal and oral cavities and suggest that WFDC2 functions in concert with related WAP domain containing proteins in epithelial host defence. WFDC2 re-expression in lung carcinomas may prove to be associated with tumour type and should be studied in further detail. Mammary gland expression of tammar WFDC2 during the course of lactation showed WFDC2 was elevated during pregnancy, reduced in early lactation and absent in mid-late lactation. WFDC2/HE4 can undergo a complex series of alternative splicing events that can potentially yield five distinct WAP domain containing protein isoforms.

Product Categories/Family for WFDC2 recombinant protein

Serine Proteases and Regulators

References

Bingle,L. et al., 2002, Oncogene. 21 (17):2768-73. Hellstrom,I. et al., 2003, Cancer Res. 63 (13):3695-700. Bingle,L. et al., 2006, Respir Res. 7 : 61. Galgano,M.T. et al., 2006, Mod Pathol.19 (6):847-53. Sharp,J.A. et al., 2007, Evol Dev. 9 (4): 378-92.

NCBI and Uniprot Product Information

• NCBI GI #: 56699495
• NCBI Accession #: NP_006094.3
• NCBI GenBank Nucleotide #: NP_006094.3
• UniProt Accession #: Q14508
• NCBI Official Full Name: WAP four-disulfide core domain protein 2
• UniProt Protein Name: WAP four-disulfide core domain protein 2
• Protein Family: WAP four-disulfide core domain protein
• UniProt Gene Name: WFDC2
• UniProt Synonym Gene Names: HE4; WAP5
• UniProt Entry Name: WFDC2_HUMAN

Uniprot Description

WFDC2: a protein that is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. This gene is expressed in pulmonary epithelial cells, and was also found to be expressed in some ovarian cancers. The encoded protein is a small secretory protein, which may be involved in sperm maturation. [provided by RefSeq, Jul 2008]

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 20q13.12

Cellular Component: extracellular space

Molecular Function: serine-type endopeptidase inhibitor activity; aspartic-type endopeptidase inhibitor activity; endopeptidase inhibitor activity; cysteine protease inhibitor activity

Biological Process: spermatogenesis; proteolysis