Immunohistochemistry (IHC)
(Immunochemical staining of rat WFDC2 in rat lung with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).)
Rabbit anti-Rat HE4 Polyclonal Antibody | anti-WFDC2 antibody
Anti-HE4 Antibody, Rabbit Polyclonal
Reactivity
Rat
Applications
ELISA, Immunohistochemistry
Purity
Protein A & Antigen Affinity
Synonyms
HE4; Polyclonal Antibody; Anti-HE4 Antibody; Rabbit Polyclonal; WAP5/WFDC2/HE4 Antibody; Rabbit PAb; Antigen Affinity Purified; WAP four-disulfide core domain 2; anti-WFDC2 antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Rat WAP5/WFDC2/HE4
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-WFDC2 antibody
ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
ELISA: 1:5000-1:10,000
IHC-P: 1:1000-1:4000
IHC-P: 1:1000-1:4000
Immunogen
Recombinant Rat WAP5/WFDC2/HE4 protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Rat WAP5/WFDC2/HE4 isoform 2 (Q8CHN3-2; Met 4-Phe 120). WAP5/WFDC2/HE4 specific IgG was purified by Rat WAP5/WFDC2/HE4 affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Immunohistochemistry (IHC)
(Immunochemical staining of rat WFDC2 in rat lung with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining of rat WFDC2 in rat lung with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining of rat WFDC2 in rat uterus with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining of rat WFDC2 in rat uterus with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).)
Related Product Information for anti-WFDC2 antibody
WAP four-disulfide core domain protein 2, also known as Epididymal secretory protein E4, Major epididymis-specific protein E4, Putative protease inhibitor WAP5, WFDC2 and HE4, is a secreted protein that contains two WAP domains. WFDC2/HE4 is a member of a family of stable 4-disulfide core proteins that are secreted at high levels. It is expressed in a number of normal tissues, including male reproductive system, regions of the respiratory tract and nasopharynx. It is highly expressed in a number of tumors cells lines, such ovarian, colon, breast, lung and renal cells lines. Initially described as being exclusively transcribed in the epididymis. WFDC2 may be a component of the innate immune defences of the lung, nasal and oral cavities and suggest that WFDC2 functions in concert with related WAP domain containing proteins in epithelial host defence. WFDC2 re-expression in lung carcinomas may prove to be associated with tumour type and should be studied in further detail. Mammary gland expression of tammar WFDC2 during the course of lactation showed WFDC2 was elevated during pregnancy, reduced in early lactation and absent in mid-late lactation. WFDC2/HE4 can undergo a complex series of alternative splicing events that can potentially yield five distinct WAP domain containing protein isoforms.
Product Categories/Family for anti-WFDC2 antibody
References
Bingle, L. et al., 2002, Oncogene. 21 (17):2768-73. Hellstrom, I. et al., 2003, Cancer Res. 63 (13):3695-700. Bingle, L. et al., 2006, Respir Res. 7 : 61. Galgano, M.T. et al., 2006, Mod Pathol.19 (6):847-53. Sharp, J.A. et al., 2007, Evol Dev. 9 (4): 378-92.
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Product Notes
The WFDC2 (Catalog #AAA8112742) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-HE4 Antibody, Rabbit Polyclonal reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's HE4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P). ELISA: 1:5000-1:10,000 IHC-P: 1:1000-1:4000. Researchers should empirically determine the suitability of the WFDC2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HE4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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