Polybromo 1 (PBRM1) Recombinant Protein | PBRM1 recombinant protein

Recombinant Polybromo 1 (PBRM1)

Cat. Num. AAA2011038

• Gene Names: PBRM1; PB1; BAF180
• Reactivity: Homo sapiens (Human)
• Applications: SDS-Page, Western Blot
• Purity: > 90%
• Synonyms: Polybromo 1 (PBRM1); Recombinant Polybromo 1 (PBRM1); PBRM1 recombinant protein

• Host: E Coli
• Reactivity: Homo sapiens (Human)
• Purity/Purification: > 90%
• Concentration: Original Concentration: 200 Ug/mL (varies by lot)
• Sequence Length: 1582

• Applicable Applications for PBRM1 recombinant protein: Positive Control, Immunogen, SDS-PAGE, WB. ; ; (May be suitable for use in other assays to be determined by the end user.)
• Source: Prokaryotic expression
• Residues: Met1~Ala306
• Tags: N-terminal His and GST Tag
• Subcellular Location: Nucleus
• Traits: Freeze-dried powder
• Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose and Proclin300.
• Predicted isoelectric point: 6.5
• Predicted Molecular Mass: 65.0 kDa
• Accurate Molecular Mass: 65kDa as determined by SDS-PAGE reducing conditions.
• [ USAGE ]: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL.; Do not vortex.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles ; Store at 2-8ºC for one month.; Aliquot and store at -80ºC for 12 months.; ; Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 93102371
• NCBI GeneID: 55193
• NCBI Accession #: NP_060783.3
• NCBI GenBank Nucleotide #: NM_018313.4
• UniProt Accession #: Q86U86; Q1RMD1; Q1RMD2; Q96MS2; Q9H2T3; Q9H2T4; Q9H2T5; Q9H301; Q9H314; A1L381; A1L382; A4FUJ7
• NCBI Official Full Name: protein polybromo-1
• NCBI Official Synonym Full Names: polybromo 1
• NCBI Official Symbol: PBRM1
• NCBI Official Synonym Symbols: PB1; BAF180
• NCBI Protein Information: protein polybromo-1; polybromo-1D; BRG1-associated factor 180
• UniProt Protein Name: Protein polybromo-1
• Protein Family: Protein polybromo
• UniProt Gene Name: PBRM1
• UniProt Synonym Gene Names: BAF180; PB1; hPB1; BAF180
• UniProt Entry Name: PB1_HUMAN

NCBI Description

This locus encodes a subunit of ATP-dependent chromatin-remodeling complexes. The encoded protein has been identified as in integral component of complexes necessary for ligand-dependent transcriptional activation by nuclear hormone receptors. Mutations at this locus have been associated with primary clear cell renal cell carcinoma. [provided by RefSeq, Feb 2012]

Uniprot Description

polybromo 1: Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Acts as a negative regulator of cell proliferation. Defects in PBRM1 are a cause of renal cell carcinoma (RCC). It is a heterogeneous group of sporadic or hereditary carcinoma derived from cells of the proximal renal tubular epithelium. It is subclassified into clear cell renal carcinoma (non-papillary carcinoma), papillary renal cell carcinoma, chromophobe renal cell carcinoma, collecting duct carcinoma with medullary carcinoma of the kidney, and unclassified renal cell carcinoma. 9 isoforms of the human protein are produced by alternative splicing.

Protein type: Tumor suppressor; DNA-binding

Chromosomal Location of Human Ortholog: 3p21

Cellular Component: kinetochore; nuclear chromosome

Molecular Function: protein binding; DNA binding; chromatin binding

Biological Process: chromatin remodeling; negative regulation of cell proliferation; mitosis; regulation of transcription, DNA-dependent; transcription, DNA-dependent; heart development; placenta development