Mouse von Willebrand factor ELISA Kit | VWF elisa kit

Mouse von Willebrand Factor, vWF ELISA Kit

Cat. Num. AAA703001

• Gene Names: Vwf; VWD; F8VWF; AI551257; C630030D09; 6820430P06Rik; B130011O06Rik
• Reactivity: Mouse
• Synonyms: von Willebrand factor; Mouse von Willebrand Factor; vWF ELISA Kit; F8VWF; VWD; coagulation factor VIII VWF; VWF elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse VWF. No significant cross-reactivity or interference between Mouse VWF and analogues was observed.
• Sequence Length: 2813

• Samples: Serum, plasma and tissue homogenates
• Detection Range: 0.78 ng/ml-50 ng/ml
• Sensitivity: 0.195 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Blood Coagulation
• Protein Biological Process 3: Blood coagulation
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for VWF elisa kit

Introduction: Willebrand factor (vWF) is a blood glycoprotein involved in hemostasis. It is deficient or defective in von Willebrand disease and is involved in a large number of other diseases, including thrombotic thrombocytopenic purpura, Heyde's syndrome, and possibly hemolytic-uremic syndrome. vWF is produced constitutively in endothelium, megakaryocytes, and subendothelial connective tissue. The basic vWF monomer is a 2050 amino acid protein. Every monomer contains a number of specific domains with a specific function. Monomers are subsequently N-glycosylated, arranged into dimers in the endoplasmic reticulum and into multimers in the Golgi apparatus by crosslinking of cysteine residues via disulfide bonds. With respect to the glycosylation, vWF is one of the few proteins that carry ABO blood group system antigens. Multimers of vWF can be extremely large, >20,000 kDa, and consist of over 80 subunits of 250 kDa each. Only the large multimers are functional. Some cleavage products that result from Vwf production are also secreted but probably serve no function. Von Willebrand factor is not an enzyme and therefore has no catalytic activity. Its primary function is binding to other proteins, particularly Factor VIII and it is important in platelet adhesion to wound sites. vWF binds to a number of cells and molecules. The most important ones are Factor VIII, collagen, platelet gpIb, other platelet receptors et al.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to vWF. Standards or samples are then added to the appropriate microtiter plate wells with a biotinconjugated antibody preparation specific for vWF and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain vWF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of vWF in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 115511022
• NCBI GeneID: 22371
• NCBI Accession #: NP_035838.3
• NCBI GenBank Nucleotide #: NM_011708.4
• UniProt Accession #: Q8CIZ8; Q60863; Q6XUV6; Q8BIU9; Q8CGN0; Q9JK16
• Molecular Weight: 309,269 Da
• NCBI Official Full Name: von Willebrand factor
• NCBI Official Synonym Full Names: Von Willebrand factor homolog
• NCBI Official Symbol: Vwf
• NCBI Official Synonym Symbols: VWD; F8VWF; AI551257; C630030D09; 6820430P06Rik; B130011O06Rik
• NCBI Protein Information: von Willebrand factor
• UniProt Protein Name: von Willebrand factor
• Protein Family: von Willebrand factor
• UniProt Gene Name: Vwf
• UniProt Synonym Gene Names: vWF
• UniProt Entry Name: VWF_MOUSE

Uniprot Description

Function: Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma By similarityManual assertion inferred from sequence similarityUniProtKB:P04275