VNN1 / Vanin-1 Recombinant Protein | VNN1 recombinant protein

Recombinant Mouse VNN1 / Vanin-1 Protein (His tag)

Cat. Num. AAA2546726

• Gene Names: Vnn1; V-1
• Purity: > 97 % as determined by SDS-PAGE
• Synonyms: VNN1 / Vanin-1; Recombinant Mouse VNN1 / Vanin-1 Protein (His tag); V-1; VNN1 recombinant protein

• Host: Human Cells
• Purity/Purification: > 97 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 512

• Application Notes: The secreted recombinant mouse VNN1 comprises 475 amino acids and has a calculated molecular mass of 53.4 kDa. As a result of glycosylation, the apparent molecular mass of rmVNN1 is approximately 60 kDa in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Leu 22
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for VNN1 recombinant protein

Background: Pantetheinase, also known as Pantetheine hydrolase, Vascular non-inflammatory molecule 1, Vanin-1, and VNN1, is a cell membrane protein which belongs to the CN hydrolase family and BTD/VNN subfamily. Vanin-1 contains one CN hydrolase domain. It is widely expressed with higher expression in spleen, kidney and blood. It is overexpressed in lesional psoriatic skin. Vanin-1 is also a member of the Vanin family of proteins which share extensive sequence similarity with each other, and also with biotinidase. The family includes secreted and membrane-associated proteins, a few of which have been reported to participate in hematopoietic cell trafficking. No biotinidase activity has been demonstrated for any of the vanin proteins, however, they possess pantetheinase activity, which may play a role in oxidative-stress response. Vanin-1 is an epithelial pantetheinase that provides cysteamine to tissue and regulates response to stress. Vanin-1 is expressed by enterocytes, and its absence limits intestinal epithelial cell production of proinflammatory signals. Vanin-1 regulates late adhesion steps of thymus homing under physiological, noninflammatory conditions. The early impact of vanin-1 deficiency on tumor induction was directly correlated to the amount of inflammation and subsequent epithelial proliferation rather than cell death rate. Vanin-1 molecule was shown to be involved in the control of thymus reconstitution following sublethal irradiation.

Description: A DNA sequence encoding the mouse VNN1 (Q9Z0K8) (Met 1-Ser 487), without the pro peptide, was fused with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 251823855
• NCBI GeneID: 22361
• NCBI Accession #: NP_035834.2
• NCBI GenBank Nucleotide #: NM_011704.3
• UniProt Accession #: Q9Z0K8; Q3TJI0; Q8VCT1; Q9DCH8
• Molecular Weight: 57,091 Da
• NCBI Official Full Name: pantetheinase
• NCBI Official Synonym Full Names: vanin 1
• NCBI Official Symbol: Vnn1
• NCBI Official Synonym Symbols: V-1
• NCBI Protein Information: pantetheinase
• UniProt Protein Name: Pantetheinase
• Protein Family: Pantetheinase
• UniProt Gene Name: Vnn1
• UniProt Synonym Gene Names: Vanin-1

Uniprot Description

VNN1: Amidohydrolase that hydrolyzes specifically one of the carboamide linkages in D-pantetheine thus recycling pantothenic acid (vitamin B5) and releasing cysteamine. Belongs to the CN hydrolase family. BTD/VNN subfamily.

Protein type: Cell adhesion; Cofactor and Vitamin Metabolism - pantothenate and CoA biosynthesis; EC 3.5.1.92; Hydrolase; Membrane protein, GPI anchor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 10|10 A4

Cellular Component: extracellular space; integral to membrane

Molecular Function: pantetheine hydrolase activity

Biological Process: acute inflammatory response; biotin metabolic process; cell-cell adhesion; central nervous system development; chronic inflammatory response; inflammatory response; innate immune response; pantothenate metabolic process; positive regulation of T cell differentiation in the thymus