Rabbit anti-Mouse, Rat STIM2 Polyclonal Antibody | anti-STIM2 antibody

RABBIT ANTI STIM2

Cat. Num. AAA223098

• Reactivity: Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Synonyms: STIM2; Polyclonal Antibody; RABBIT ANTI STIM2; anti-STIM2 antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item recognises an internal region of stromal interaction molecule-2 (STIM2), a ubiquitously expressed type I membrane protein, of which at least three isoforms have been identified. ; ; In T-lymphocytes and other haematopoietic cells, the mechanism for increasing intracellular Ca2+, following antigen mediated T-cell activation, is through the store-operated Ca2+release-activated Ca2+ (CRAC) channels. STIM1 is reported to activate CRAC channels upon Ca2+depletion. Whereas, STIM2 acts to inhibit the STIM1-mediated store-operated Ca2+entry, and forms complexes with STIM1, suggesting that these molecules may play a co-ordinated role in controlling Ca2+entry.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0 mg/ml (varies by lot)
• Sequence Length: 599

• Applicable Applications for anti-STIM2 antibody: Immunohistology Paraffin*, Western Blot (WB)
• Application Notes: Western Blot: This item detects a band of approximately 105kDa in A20 cell lysates ; Immunohistology - Paraffin: Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.; Western Blotting: Minimum Dilution: 0.5; Maximum Dilution: 1.0ug/ml
• Perservative Stabilisers: 0.02% Sodium Azide
• Immunogen: A 17 amino acid peptide located near the centre of human STIM2.; Buffer Solution
• Antiserum Preparation: Antisera to human STIM2 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Western blot analysis of whole cell lysate from A20 mouse B-cell lymphoma cells probed with Rabbit anti Human STIM2 (MBS223098) at 0.5(A), 1(B) and 2(C) ug/ml)

Testing Data #2

(Formalin fixed, paraffin embedded human spleen stained with Rabbit anti Human STIM2 (MBS223098) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0))

Related Product Information for anti-STIM2 antibody

MBS223098 recognises an internal region of stromal interaction molecule-2 (STIM2), a ubiquitously expressed type I membrane protein, of which at least three isoforms have been identified. In T-lymphocytes and other haematopoietic cells, the mechanism for increasing intracellular Ca2+, following antigen mediated T-cell activation, is through the store-operated Ca2+release-activated Ca2+ (CRAC) channels. STIM1 is reported to activate CRAC channels upon Ca2+depletion. Whereas, STIM2 acts to inhibit the STIM1-mediated store-operated Ca2+entry, and forms complexes with STIM1, suggesting that these molecules may play a co-ordinated role in controlling Ca2+entry.

NCBI and Uniprot Product Information

• NCBI GI #: 281182836
• NCBI GeneID: 57620
• NCBI Accession #: NP_001162588.1
• NCBI GenBank Nucleotide #: NM_001169117.1
• UniProt Accession #: Q9P246; Q96BF1; Q9BQH2; Q9H8R1; A6H8L7; B7ZVY0
• Molecular Weight: 68,026 Da
• NCBI Official Full Name: stromal interaction molecule 2 isoform 3
• NCBI Official Synonym Full Names: stromal interaction molecule 2
• NCBI Official Symbol: STIM2
• NCBI Protein Information: stromal interaction molecule 2
• UniProt Protein Name: Stromal interaction molecule 2
• Protein Family: Stromal interaction molecule
• UniProt Gene Name: STIM2
• UniProt Synonym Gene Names: KIAA1482
• UniProt Entry Name: STIM2_HUMAN

NCBI Description

This gene is a member of the stromal interaction molecule (STIM) family and likely arose, along with related family member STIM1, from a common ancestral gene. The encoded protein functions to regulate calcium concentrations in the cytosol and endoplasmic reticulum, and is involved in the activation of plasma membrane Orai Ca(2+) entry channels. This gene initiates translation from a non-AUG (UUG) start site. A signal peptide is cleaved from the resulting protein. Multiple transcript variants result from alternative splicing. [provided by RefSeq, Dec 2009]

Uniprot Description

STIM2: Plays a role in mediating store-operated Ca(2+) entry (SOCE), a Ca(2+) influx following depletion of intracellular Ca(2+) stores. Functions as a highly sensitive Ca(2+) sensor in the endoplasmic reticulum which activates both store-operated and store-independent Ca(2+)-influx. Regulates basal cytosolic and endoplasmic reticulum Ca(2+) concentrations. Upon mild variations of the endoplasmic reticulum Ca(2+) concentration, translocates from the endoplasmic reticulum to the plasma membrane where it probably activates the Ca(2+) release-activated Ca(2+) (CRAC) channels ORAI1, ORAI2 and ORAI3. May inhibit STIM1-mediated Ca(2+) influx. Oligomer with STIM1. Interacts with ORAI1. Expressed in all tissues and tumor cell lines examined.

Protein type: Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 4p15.2

Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum; integral to membrane; plasma membrane

Molecular Function: store-operated calcium channel activity; protein binding; calcium channel regulator activity; calcium ion binding

Biological Process: cellular calcium ion homeostasis; activation of store-operated calcium channel activity; positive regulation of calcium ion transport