Vanin 1 (VNN1) Recombinant Protein | VNN1 recombinant protein

Recombinant Vanin 1 (VNN1)

Cat. Num. AAA2009679

• Gene Names: Vnn1; V-1
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Vanin 1 (VNN1); Recombinant Vanin 1 (VNN1); VNN1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-V EGQYYLQICI LLKCKTTNLR TCGSSVDTAF TRFEMFSLSG TFGTRYVFPE VLLSEVKLAP GEFQVSSDGR LVSLKPTSGP VLTIGLFGRL YGKDWASNAS SDFIAHSLII MLI
• Sequence Length: 512

• Applicable Applications for VNN1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Val390~Ile503 (Accession # Q9Z0K8) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

NCBI and Uniprot Product Information

• NCBI GI #: 251823855
• NCBI GeneID: 22361
• NCBI Accession #: NP_035834.2
• NCBI GenBank Nucleotide #: NM_011704.3
• UniProt Accession #: Q9Z0K8; Q3TJI0; Q8VCT1; Q9DCH8
• Molecular Weight: 14.0kDa
• NCBI Official Full Name: pantetheinase
• NCBI Official Synonym Full Names: vanin 1
• NCBI Official Symbol: Vnn1
• NCBI Official Synonym Symbols: V-1
• NCBI Protein Information: pantetheinase; vanin-1; pantetheine hydrolase; vascular non-inflammatory molecule 1
• UniProt Protein Name: Pantetheinase
• Protein Family: Pantetheinase
• UniProt Gene Name: Vnn1
• UniProt Synonym Gene Names: Vanin-1
• UniProt Entry Name: VNN1_MOUSE

Uniprot Description

VNN1: Amidohydrolase that hydrolyzes specifically one of the carboamide linkages in D-pantetheine thus recycling pantothenic acid (vitamin B5) and releasing cysteamine. Belongs to the CN hydrolase family. BTD/VNN subfamily.

Protein type: Cell adhesion; Motility/polarity/chemotaxis; Membrane protein, integral; EC 3.5.1.92; Cofactor and Vitamin Metabolism - pantothenate and CoA biosynthesis; Membrane protein, GPI anchor; Hydrolase

Cellular Component: extracellular space; membrane; plasma membrane; integral to membrane

Molecular Function: hydrolase activity; pantetheine hydrolase activity; hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds; hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides

Biological Process: positive regulation of T cell differentiation in the thymus; cell-cell adhesion; chronic inflammatory response; nitrogen compound metabolic process; pantothenate metabolic process; innate immune response; inflammatory response; acute inflammatory response