SDS-PAGE
(Figure annotation denotes ug of protein loaded and % gel used.)
VHL recombinant protein
VHL Ag, betadomain Ag(His tag)
Applications
SDS-Page
Purity
> 95% pure by SDS-PAGE
Synonyms
VHL; VHL Ag; betadomain Ag(His tag); HRCA1 protein; RCA1 protein; VHL 1 protein; VHL1 protein; G7 protein protein; Von Hippel-Lindau tumor suppressor isoform 1 protein; von Hippel Lindau tumor suppressor protein; Von Hippel-Lindau tumor suppressor isoform 1 pVHL protein; RCA 1 protein; HRCA 1 protein; VHLH protein; von Hippel Lindau syndrome protein; Elongin binding protein protein; Von Hippel Lindau disease tumor suppressor protein; VHL protein; VHL recombinant protein
Host
E.coli
Purity/Purification
> 95% pure by SDS-PAGE
Form/Format
Supplied as a liquid in PBS, pH 7.4, containing 2mM EDTA
Concentration
1 mg/ml (by Bradford) (varies by lot)
Sequence
1-154 amino acids: MGSSHHHHHH SSGLVPRGSH MPRRAENWDE AEVGAEEAGV EEYGPEEDGG EESGAEESGP EESGPEELGA EEEMEAGRPR PVLRSVNSRE PSQVIFCNRS PRVVLPVWLN FDGEPQPYPT LPPGTGRRIH SYRGHLWLFR DAGTHDGLLV NQTELFVPSL NVDGQPIFAN ITLP
Applicable Applications for VHL recombinant protein
SDS-PAGE
Protein Type
Recombinant
Biological Significance
Von Hippel-Lindau disease(VHL) is a dominant inherited syndrome characterized by the predisposition to develop various kinds of benign and malignant tumors, including clear cell renal carcinomas, pheochromocytomas and hemangioblastomas of the central nervous system and retina. VHL syndrome is caused by germline mutation in the VHL tumor suppressor, and VHL tumors are associated with loss or mutation of the remaining wild-type allele. VHL has two domains: a roughly 100-residue NH2-terminal domain rich in beta sheet (beta-domain) and a smaller alpha-helical domain (alpha-domain), held together by two linkers and a polar interface. VHL protein is also involved in the degradation of hypoxia-inducible factor (HIF). VHL beta-domain(1-154aa) was overexpressed in E Coli and purified by using conventional chromatography techniques
Biohazard
Recombinant protein with His Tag. Use standard laboratory precautions when handling this material.
Species
Human
Preparation and Storage
4°C short term, -20°C to -70°C long term. Avoid repeated freeze-thaws.
SDS-PAGE
(Figure annotation denotes ug of protein loaded and % gel used.)
SDS-PAGE
(Figure annotation denotes ug of protein loaded and % gel used.)
Related Product Information for VHL recombinant protein
Purified recombinant Human VHL protein, betadomain protein
Product Categories/Family for VHL recombinant protein
Similar Products
Product Notes
The VHL (Catalog #AAA835911) is a Recombinant Protein produced from E.coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's VHL can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the VHL for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: 1-154 amino acids: MGSSHHHHHH SSGLVPRGSH MPRRAENWDE AEVGAEEAGV EEYGPEEDGG EESGAEESGP EESGPEELGA EEEMEAGRPR PVLRSVNSRE PSQVIFCNRS PRVVLPVWLN FDGEPQPYPT LPPGTGRRIH SYRGHLWLFR DAGTHDGLLV NQTELFVPSL NVDGQPIFAN ITLP. It is sometimes possible for the material contained within the vial of "VHL, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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