VEGF B Recombinant Protein | VEGFB recombinant protein

VEGF B Protein, Human, Recombinant (hFc Tag)

Cat. Num. AAA8118639

• Gene Names: VEGFB; VRF; VEGFL
• Purity: >95 % as determined by SDS-PAGE
• Synonyms: VEGF B; VEGF B Protein; Human; Recombinant (hFc Tag); Human VEGF-B/VEGFB Protein (Fc Tag); vascular endothelial growth factor B; VEGFL Protein; VRF Protein; VEGFB recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Sequence Positions: 446aa
• Sequence: Pro22-Ala207

• Species: Human
• Tag: hFc
• Endotoxin: <1.0EU per ug protein as determined by the LAL method
• Predicted N Terminal: Glu
• Protein Construction: |A DNA sequence encoding the human VEGFB (NP_003368.1) (Pro22-Ala207) was expressed with the Fc region of human IgG1 at the N-terminus
• Activity: Measured by its binding ability in a functional ELISA.Immobilized human S4-Fc3L3-VEGFB at 10ug/ml (100uL/well) can bind biotinylated human NRP1-Fc (Cat:10011-H02H). The EC50 of biotinylated human NRP1-Fc (Cat:10011-H02H) is 0.03-0.07ug/mL.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; Shipping: They are shipped out with blue ice pack

Testing Data

Related Product Information for VEGFB recombinant protein

Vascular endothelial growth factor-B (VEGF-B) is closely related to VEGF-A, an effector of blood vessel growth during development and disease and a strong candidate for angiogenic therapies. In detail, VEGFB can positively prevent the Ang II-induced rising in the size of cardiomyocyte as well as reduce Ang II-induced mRNA and protein levels of beta-MHC (beta-myosin heavy chain), BNP (brain natriuretic peptide), and ANP (atrial natriuretic peptide). Moreover, VEGFB can regulate the decline of the Ang II-induced rising in Ca2+.

NCBI and Uniprot Product Information

• NCBI GI #: 4507887
• NCBI GeneID: 7423
• NCBI Accession #: NP_003368.1
• NCBI GenBank Nucleotide #: NM_003377.4
• UniProt Accession #: P49765; Q16528
• Molecular Weight: 21,602 Da
• NCBI Official Full Name: vascular endothelial growth factor B isoform VEGFB-186
• NCBI Official Synonym Full Names: vascular endothelial growth factor B
• NCBI Official Symbol: VEGFB
• NCBI Official Synonym Symbols: VRF; VEGFL
• NCBI Protein Information: vascular endothelial growth factor B; VEGF-related factor
• UniProt Protein Name: Vascular endothelial growth factor B
• Protein Family: Vascular endothelial growth factor
• UniProt Gene Name: VEGFB
• UniProt Synonym Gene Names: VRF; VEGF-B; VRF
• UniProt Entry Name: VEGFB_HUMAN

NCBI Description

This gene encodes a member of the PDGF (platelet-derived growth factor)/VEGF (vascular endothelial growth factor) family. The VEGF family members regulate the formation of blood vessels and are involved in endothelial cell physiology. This member is a ligand for VEGFR-1 (vascular endothelial growth factor receptor 1) and NRP-1 (neuropilin-1). Studies in mice showed that this gene was co-expressed with nuclear-encoded mitochondrial genes and the encoded protein specifically controlled endothelial uptake of fatty acids. Alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Sep 2011]

Uniprot Description

VEGFB: Growth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis. Homodimer; disulfide-linked. Can also form heterodimer with VEGF. Expressed in all tissues except liver. Highest levels found in heart, skeletal muscle and pancreas. Belongs to the PDGF/VEGF growth factor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; Cytokine; Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: extracellular space; membrane; extracellular region

Molecular Function: heparin binding; protein binding; protein homodimerization activity; growth factor activity; protein heterodimerization activity; vascular endothelial growth factor receptor 1 binding; chemoattractant activity

Biological Process: response to drug; protein amino acid O-linked glycosylation; platelet activation; positive regulation of vascular permeability; positive regulation of vascular endothelial growth factor receptor signaling pathway; induction of positive chemotaxis; positive regulation of protein kinase B signaling cascade; positive regulation of peptidyl-tyrosine phosphorylation; platelet degranulation; positive chemotaxis; positive regulation of cell division; positive regulation of endothelial cell proliferation; angiogenesis; negative regulation of neuron apoptosis; blood coagulation; vascular endothelial growth factor receptor signaling pathway; cardiac muscle contraction; negative regulation of apoptosis