VAPA Human Recombinant Protein | VAPA recombinant protein

VAPA, Recombinant Human (hVAP-33, VAP-33, VAP-A, VAP33)

Cat. Num. AAA637377

• Gene Names: VAPA; VAP-A; VAP33; VAP-33; hVAP-33
• Applications: Western Blot
• Purity: Affinity Purified; ~85% by SDS-PAGE.
• Synonyms: VAPA Human; VAPA; Recombinant Human (hVAP-33; VAP-33; VAP-A; VAP33); VAPA recombinant protein

• Host: E Coli
• Purity/Purification: Affinity Purified; ~85% by SDS-PAGE.
• Form/Format: Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 10% glycerol, 1mM DTT.
• Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMAS ASGAMAKHEQ ILVLDPPTDL KFKGPFTDVV TTNLKLRNPS DRKVCFKVKT TAPRRYCVRP NSGIIDPGST VTVSVMLQPF DYDPNEKSKH KFMVQTIFAP PNTSDMEAVW KEAKPDELMD SKLRCVFEMP NENDKLNDME PSKAVPLNAS KQDGPMPKPH SVSLNDTETR KLMEECKRLQ GEMMKLSEEN RHLRDEGLRL RKVAHSDKPG STSTASFRDN VTSP

• Applicable Applications for VAPA recombinant protein: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.
• Endotoxin Level: 1EU/1ug (LAL).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

SDS-Page

(15% SDS-PAGE (3ug))

Related Product Information for VAPA recombinant protein

VAPA is a type IV membrane protein, present in the plasma membrane, intracellular vesicles and may also be associated with the cytoskeleton. This protein is a SNARE regulator with high levels of expression in the intestine during late embryogenesis and early neonatal development. VAPA may function in vesicle trafficking, membrane fusion, protein complex assembly and cell motility.

Recombinant VAPA protein was expressed in E.coli and purified by using conventional chromatography techniques.

Product Categories/Family for VAPA recombinant protein

Molecular Biology; MB-Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 94721252
• NCBI GeneID: 9218
• NCBI Accession #: NP_919415.2
• NCBI GenBank Nucleotide #: NM_194434.2
• UniProt Accession #: Q9P0L0; O75453; Q5U0E7; Q9UBZ2; A6NDZ0; D3DUI3
• Molecular Weight: 29.8kD
• NCBI Official Full Name: vesicle-associated membrane protein-associated protein A isoform 2
• NCBI Official Synonym Full Names: VAMP (vesicle-associated membrane protein)-associated protein A, 33kDa
• NCBI Official Symbol: VAPA
• NCBI Official Synonym Symbols: VAP-A; VAP33; VAP-33; hVAP-33
• NCBI Protein Information: vesicle-associated membrane protein-associated protein A; VAMP-A; VAMP-associated protein A; 33 kDa VAMP-associated protein
• UniProt Protein Name: Vesicle-associated membrane protein-associated protein A
• Protein Family: Virulence-associated protein
• UniProt Gene Name: VAPA
• UniProt Synonym Gene Names: VAP33; VAMP-A; VAMP-associated protein A; VAP-A; VAP-33
• UniProt Entry Name: VAPA_HUMAN

NCBI Description

The protein encoded by this gene is a type IV membrane protein. It is present in the plasma membrane and intracellular vesicles. It may also be associated with the cytoskeleton. This protein may function in vesicle trafficking, membrane fusion, protein complex assembly and cell motility. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

VAPA: May play a role in vesicle trafficking. Belongs to the VAMP-associated protein (VAP) (TC 9.B.17) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 18p11.22

Cellular Component: microtubule cytoskeleton; Golgi membrane; endoplasmic reticulum membrane; tight junction; perinuclear region of cytoplasm; endoplasmic reticulum; integral to membrane; plasma membrane; vesicle

Molecular Function: protein domain specific binding; signal transducer activity; protein binding; FFAT motif binding; protein heterodimerization activity; microtubule binding

Biological Process: endoplasmic reticulum organization and biogenesis; cell death; ER to Golgi vesicle-mediated transport; positive regulation of I-kappaB kinase/NF-kappaB cascade; sphingolipid metabolic process; sphingolipid biosynthetic process; signal transduction; neurite development