Rabbit anti-Human GSTA3 Polyclonal Antibody | anti-GSTA3 antibody

GSTA3 Antibody

Cat. Num. AAA9401311

• Gene Names: GSTA3; GTA3; GSTA3-3
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Antigen affinity purification.
• Synonyms: GSTA3; Polyclonal Antibody; GSTA3 Antibody; GTA3; GSTA3-3; anti-GSTA3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total GSTA3 protein.
• Purity/Purification: Antigen affinity purification.
• Form/Format: Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 222

• Applicable Applications for anti-GSTA3 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western blotting: 1:500-1:2000; Immunohistochemistry: 1:50-1:200
• Immunogen Type: Recombinant Protein
• Immunogen Description: Full length fusion protein
• Target Name: GSTA3
• Preparation and Storage: Store at -20 degree C

Testing Data

(Gel: 10%SDS-PAGE Lysates (from left to right): Human testis tissue and HepG2 cell Amount of lysate: 40ug per lane Primary antibody: 1/250 dilution Secondary antibody dilution: 1/8000 Exposure time: 10 seconds)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue using at dilution 1/30.)

Related Product Information for anti-GSTA3 antibody

Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. These enzymes are involved in cellular defense against toxic, carcinogenic, and pharmacologically active electrophilic compounds. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-tranferase belonging to the alpha class genes that are located in a cluster mapped to chromosome 6. Genes of the alpha class are highly related and encode enzymes with glutathione peroxidase activity. However, during evolution, this alpha class gene diverged accumulating mutations in the active site that resulted in differences in substrate specificity and catalytic activity.

Product Categories/Family for anti-GSTA3 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 24430144
• NCBI GeneID: 2940
• NCBI Accession #: NP_000838.3
• NCBI GenBank Nucleotide #: NM_000847.4
• UniProt Accession #: Q16772; O43468; Q068V6; Q8WWA8; Q9H415
• Molecular Weight: 25,302 Da
• NCBI Official Full Name: glutathione S-transferase A3
• NCBI Official Synonym Full Names: glutathione S-transferase alpha 3
• NCBI Official Symbol: GSTA3
• NCBI Official Synonym Symbols: GTA3; GSTA3-3
• NCBI Protein Information: glutathione S-transferase A3
• UniProt Protein Name: Glutathione S-transferase A3
• Protein Family: Glutathione S-transferase
• UniProt Gene Name: GSTA3
• UniProt Entry Name: GSTA3_HUMAN

NCBI Description

Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. These enzymes are involved in cellular defense against toxic, carcinogenic, and pharmacologically active electrophilic compounds. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-tranferase belonging to the alpha class genes that are located in a cluster mapped to chromosome 6. Genes of the alpha class are highly related and encode enzymes with glutathione peroxidase activity. However, during evolution, this alpha class gene diverged accumulating mutations in the active site that resulted in differences in substrate specificity and catalytic activity. The enzyme encoded by this gene catalyzes the double bond isomerization of precursors for progesterone and testosterone during the biosynthesis of steroid hormones. An additional transcript variant has been identified, but its full length sequence has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

GSTA3: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Catalyzes isomerization reactions that contribute to the biosynthesis of steroid hormones. Efficiently catalyze obligatory double-bond isomerizations of delta(5)-androstene-3,17-dione and delta(5)- pregnene-3,20-dione, precursors to testosterone and progesterone, respectively. Belongs to the GST superfamily. Alpha family.

Protein type: Other Amino Acids Metabolism - glutathione; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Transferase; EC 2.5.1.18; Xenobiotic Metabolism - metabolism by cytochrome P450

Chromosomal Location of Human Ortholog: 6p12.1

Cellular Component: cytosol

Molecular Function: glutathione transferase activity

Biological Process: glutathione metabolic process; xenobiotic metabolic process