VAMP8 recombinant protein

VAMP8, 1-76aa, Human, His tag, E Coli

Cat. Num. AAA203171

• Gene Names: VAMP8; EDB; VAMP-8
• Applications: SDS-Page
• Purity: > 90% by SDS PAGE
• Synonyms: VAMP8; 1-76aa; Human; His tag; E Coli; Vesicle-associated membrane protein 8; Endobrevin; EDB; Vesicle-associated membrane protein 8 VAMP 5; VAMP 8; VAMP-8; VAMP5; Vesicle associated membrane protein 8.; VAMP8 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS PAGE
• Form/Format: Liquid in 20mM Tris pH8.5. 0.1mM PMSF, 0.2M NaCl, 50% glycerol, 0.1M Imidazole
• Concentration: 0.25mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MEEASEGGGN DRVRNLQSEV EGVKNIMTQN VERILARGEN LEHLRNKTED LEATSEHFKT TSQKVARKFW WKNVKM
• Sequence Length: 100

• Applicable Applications for VAMP8 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for VAMP8 recombinant protein

Vehicle-associated membrane (VAMP8),also known as endobrevin, is the main components of a SNARE complex involved in the docking and fusion of synaptic vesicles with the presynaptic membrane. This protein plays a role in regulated enzyme secretion in pancreatic acinar cells and is involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells. It is required for dense-granule secretion in platelets. Recombinant VAMP8 protein was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for VAMP8 recombinant protein

Neuroscience

References

Polgar J., et al. (2002) Blood. 100 (3):1081-3.; Ren Q., et al. (2007) Mol Biol Cell. 18 (1):24-33;

NCBI and Uniprot Product Information

• NCBI GI #: 14043026
• NCBI GeneID: 8673
• NCBI Accession #: NP_003752
• NCBI GenBank Nucleotide #: NM_003761.4
• UniProt Accession #: Q9BV40; O60625; Q53SP9; Q6IB09
• Molecular Weight: 10.9kDa (96aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: vesicle-associated membrane protein 8
• NCBI Official Synonym Full Names: vesicle associated membrane protein 8
• NCBI Official Symbol: VAMP8
• NCBI Official Synonym Symbols: EDB; VAMP-8
• NCBI Protein Information: vesicle-associated membrane protein 8
• UniProt Protein Name: Vesicle-associated membrane protein 8
• Protein Family: Vesicle-associated membrane protein
• UniProt Gene Name: VAMP8
• UniProt Synonym Gene Names: EDB

NCBI Description

This gene encodes an integral membrane protein that belongs to the synaptobrevin/vesicle-associated membrane protein subfamily of soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs). The encoded protein is involved in the fusion of synaptic vesicles with the presynaptic membrane.[provided by RefSeq, Jun 2010]

Uniprot Description

SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. VAMP8 is a SNARE involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane via its interaction with the STX17-SNAP29 binary t-SNARE complex (PubMed:23217709, PubMed:25686604). Also required for dense-granule secretion in platelets (PubMed:12130530). Plays also a role in regulated enzyme secretion in pancreatic acinar cells (). Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells (). Involved in the homotypic fusion of early and late endosomes ().