Podoplanin Active Protein | PDPN active protein

Podoplanin Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122641

• Gene Names: Pdpn; T1a; Gp38; OTS-8; T1alpha; RANDAM-2; T1-alpha
• Purity: >92% as determined by SDS-PAGE
• Synonyms: Podoplanin; Podoplanin Protein; Mouse; Recombinant (His Tag); Mouse Podoplanin/PDPN Protein (His Tag); Gp38 Protein; OTS-8 Protein; RANDAM-2 Protein; T1-alpha Protein; T1a Protein; T1alpha Protein; podoplanin; PDPN active protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >92% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH7.4, 10% gly. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Lys133

• Species: Mouse
• Activity: Measured by its ability to bind recombinant CLEC1B-His/Biotin in a functional ELISA.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Gly 23
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mouse PDPN N-terminal fragment (NP_034459.2)(Met 1-Lys 133) was expressed, with a C-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for PDPN active protein

Background: Podoplanin, also known as PDPN, is a type-I integral membrane glycoprotein with diverse distribution in human tissues. The physiological function of this protein may be related to its mucin-type character. The homologous protein in other species has been described as a differentiation antigen and influenza-virus receptor. The specific function of this protein has not been determined. Alternatively spliced transcript variants encoding different isoforms have been identified.PDPN is a mucin-type glycoprotein negatively charged by extensive O-glycosylation and a high content of sialic acid, which expresses the adhesive property. It is selectively expressed in lymphatic endothelium as well as lymphangiomas, Kaposi sarcomas, and in a subset of angiosarcomas with probable lymphatic differentiation. PDPN may contribute to form odontoblastic fiber or function as the anchorage to the tooth development and in proliferating epithelial cells of cervical loop and apical bud. The intensity of podoplanin expression is negatively correlated with the expression of CD34 and factor VIII. Podoplanin would be useful as a test marker for epithelioid hemangioendothelioma in liver tumors.

References

Kimura, N. et al., 2005, Pathol Int. 55 (2): 83-86. Orda³a+/-ez, N.G., 2006, Adv Anat Pathol. 13 (2): 83-88. Wicki, A. et al., 2007, Br. J. Cancer. 96 (1): 1-5. Fujii,T. et al., 2008, Mod Pathol. 21 (2): 125-130. Sawa,Y. et al., 2008, Acta Histochem Cytochem. 41 (5):121-126. Kaddu, S. et al., 2009, Am J Dermatopathol. 31 (2): 137-139.

NCBI and Uniprot Product Information

• NCBI GI #: 113462005
• NCBI GeneID: 14726
• NCBI Accession #: NP_034459.2
• NCBI GenBank Nucleotide #: NM_010329.2
• UniProt Accession #: Q62011; Q546R8; Q61612; A2A8J3
• Molecular Weight: 18,233 Da
• NCBI Official Full Name: podoplanin
• NCBI Official Synonym Full Names: podoplanin
• NCBI Official Symbol: Pdpn
• NCBI Official Synonym Symbols: T1a; Gp38; OTS-8; T1alpha; RANDAM-2; T1-alpha
• NCBI Protein Information: podoplanin; aggrus; PA2.26 antigen; glycoprotein 38; transmembrane glycoprotein E11
• UniProt Protein Name: Podoplanin
• Protein Family: Podoplanin
• UniProt Gene Name: Pdpn
• UniProt Synonym Gene Names: Gp38; Ots8; Gp38; T1A

Uniprot Description

May be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. Required for normal lung cell proliferation and alveolus formation at birth. Induces platelet aggregation. Does not have any effect on folic acid or amino acid transport. Does not function as a water channel or as a regulator of aquaporin-type water channels.