SDS-Page
uPK3A recombinant protein
uPK3A, 19-207aa, Human, His tag, E Coli
Gene Names
UPK3A; UP3A; UPK3; UPIII; UPIIIA
Applications
SDS-Page
Purity
> 90% by SDS-PAGE
Synonyms
uPK3A; 19-207aa; Human; His tag; E Coli; uroplakin-3a; uPK3; uPK3A recombinant protein
Host
E Coli
Purity/Purification
> 90% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 150mM NaCl
Concentration
0.25mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMVNLQP QLASVTFATN NPTLTTVALE KPLCMFDSKE ALTGTHEVYL YVLVDSAISR NASVQDSTNT PLGSTFLQTE GGRTGPYKAV AFDLIPCSDL PSLDAIGDVS KASQILNAYL VRVGANGTCL WDPNFQGLCN PPLSAATEYR FKYVLVNMST GLVEDQTLWS DPIRTNQLTP YSTIDTWPGR RSGG
Sequence Length
287
Applicable Applications for uPK3A recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for uPK3A recombinant protein
uPK3A belongs to the uroplakin-3 family. uroplakins (uP) are key components of the urothelium. They are transmembrane proteins that pass the lipid bilayer once (uPII, uPIIIa, and uPIIIb) or four times (uPIa and uPIb; both belonging to the 'tetraspanin' family). All have large luminal/extracellular domains, but only uPIIIa and uPIIIb have significant cytoplasmic portions in their C-termini. uPK3A is a highly specific and moderately sensitive immunohistochemical marker for primary and metastatic urothelial carcinomas. Mutations in this gene may be associated with renal adysplasia Recombinant human uPK3A protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
Product Categories/Family for uPK3A recombinant protein
References
Shapiro, E., et al. (2000) J. urol. 164: 1048-1051.; Wu, X.R., et al. (1994) J. Biol. Chem. 269:13716-13724.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
23.1kDa (214aa), confirmed by MALDI-TOF
NCBI Official Full Name
uroplkin 3
NCBI Official Synonym Full Names
uroplakin 3A
NCBI Official Symbol
UPK3A
NCBI Official Synonym Symbols
UP3A; UPK3; UPIII; UPIIIA
NCBI Protein Information
uroplakin-3a
UniProt Protein Name
Uroplakin-3a
Protein Family
UniProt Gene Name
UPK3A
UniProt Synonym Gene Names
UPK3; UP3a; UPIII
NCBI Description
This gene encodes a member of the uroplakin family, a group of transmembrane proteins that form complexes on the apical surface of the bladder epithelium. Mutations in this gene may be associated with renal adysplasia. Alternatively spliced transcript variants have been described.[provided by RefSeq, Nov 2009]
Uniprot Description
Component of the asymmetric unit membrane (AUM); a highly specialized biomembrane elaborated by terminally differentiated urothelial cells. May play an important role in AUM-cytoskeleton interaction in terminally differentiated urothelial cells. It also contributes to the formation of urothelial glycocalyx which may play an important role in preventing bacterial adherence ().
Research Articles on uPK3A
Similar Products
Product Notes
The uPK3A upk3a (Catalog #AAA204417) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's uPK3A can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the uPK3A upk3a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MGSHMVNLQP QLASVTFATN NPTLTTVALE KPLCMFDSKE ALTGTHEVYL YVLVDSAISR NASVQDSTNT PLGSTFLQTE GGRTGPYKAV AFDLIPCSDL PSLDAIGDVS KASQILNAYL VRVGANGTCL WDPNFQGLCN PPLSAATEYR FKYVLVNMST GLVEDQTLWS DPIRTNQLTP YSTIDTWPGR RSGG. It is sometimes possible for the material contained within the vial of "uPK3A, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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