Rat Uroplakin 3A (UPK3A) ELISA Kit | UPK3A elisa kit

Rat Uroplakin 3A (UPK3A) ELISA Kit

Cat. Num. AAA2705855

• Gene Names: UPK3A; UP3A; UPK3; UPIII; UPIIIA
• Reactivity: Rat
• Synonyms: Uroplakin 3A (UPK3A); Rat Uroplakin 3A (UPK3A) ELISA Kit; UPIII; UPK3; UPK3A elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Uroplakin 3A (UPK3A).; No significant cross-reactivity or interference between Uroplakin 3A (UPK3A) and analogues was observed.
• Sequence Length: 166

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates and Other Biological Fluids
• Detection Range: 15.62-1000pg/mL
• Sensitivity: < 6.5pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Uroplakin 3A (UPK3A) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Uroplakin 3A (UPK3A) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for UPK3A elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Uroplakin 3A (UPK3A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Uroplakin 3A (UPK3A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Uroplakin 3A (UPK3A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Uroplakin 3A (UPK3A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 266456217
• NCBI GeneID: 7380
• NCBI Accession #: NP_001161046.1
• NCBI GenBank Nucleotide #: NM_001167574.1
• UniProt Accession #: O75631; O60261; Q32N05; Q5TII6; B0QY25
• Molecular Weight: 17,672 Da
• NCBI Official Full Name: uroplakin-3a isoform 2
• NCBI Official Synonym Full Names: uroplakin 3A
• NCBI Official Symbol: UPK3A
• NCBI Official Synonym Symbols: UP3A; UPK3; UPIII; UPIIIA
• NCBI Protein Information: uroplakin-3a
• UniProt Protein Name: Uroplakin-3a
• Protein Family: Uroplakin
• UniProt Gene Name: UPK3A
• UniProt Synonym Gene Names: UPK3; UP3a; UPIII

NCBI Description

This gene encodes a member of the uroplakin family, a group of transmembrane proteins that form complexes on the apical surface of the bladder epithelium. Mutations in this gene may be associated with renal adysplasia. Alternatively spliced transcript variants have been described.[provided by RefSeq, Nov 2009]

Uniprot Description

UPK3A: Component of the asymmetric unit membrane (AUM); a highly specialized biomembrane elaborated by terminally differentiated urothelial cells. May play an important role in AUM-cytoskeleton interaction in terminally differentiated urothelial cells. It also contributes to the formation of urothelial glycocalyx which may play an important role in preventing bacterial adherence. Defects in UPK3A are a cause of renal adysplasia (RADYS); also known as renal agenesis or renal aplasia. Renal agenesis refers to the absence of one (unilateral) or both (bilateral) kidneys at birth. Bilateral renal agenesis belongs to a group of perinatally lethal renal diseases, including severe bilateral renal dysplasia, unilateral renal agenesis with contralateral dysplasia and severe obstructive uropathy. Belongs to the uroplakin-3 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 22q13.31

Cellular Component: apical plasma membrane; endoplasmic reticulum membrane; integral component of membrane

Molecular Function: protein binding

Biological Process: cell morphogenesis; epithelial cell differentiation; kidney development; potassium ion homeostasis; sodium ion homeostasis; urea transport; urinary bladder development; water transport

Disease: Renal Hypodysplasia/aplasia 1