SDS-Page
Uracil DNA Glycosylase Recombinant Protein | UNG recombinant protein
Recombinant Uracil DNA Glycosylase (UNG)
Gene Names
UNG; ATUNG; UNG; uracil dna glycosylase
Applications
SDS-Page, Western Blot
Purity
> 95%
Synonyms
Uracil DNA Glycosylase; Recombinant Uracil DNA Glycosylase (UNG); UNG recombinant protein
Host
E.coli
Purity/Purification
> 95%
Form/Format
20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.
Concentration
Original Concentration: 300ug/mL (varies by lot)
Sequence
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MIGQKTLYSF FSPSPARKRH APSPEPAVQG TGVAGVPEES GDAAAIPAKKAPAGQEEPGT PPSSPLSAEQ LDRIQRNKAA ALLRLAARNV PVGFGESWKKHLSGEFGKPY FIKLMGFVAE ERKHYTVYPP PHQVFTWTQM CDIKDVKVVILGQDPYHGPN QAHGLCFSVQ RPVPPPPSLE NIYKELSTDI EDFVHPGHGDLSGWAKQGVL LLNAVLTVRA HQANSHKERG WEQFTDAVVS WLNQNSNGLVFLLWGSYAQK KGSAIDRKRH HVLQTAHPSP LSVYRGFFGC RHFSKTNELLQKSGKKPIDW KEL
MIGQKTLYSF FSPSPARKRH APSPEPAVQG TGVAGVPEES GDAAAIPAKKAPAGQEEPGT PPSSPLSAEQ LDRIQRNKAA ALLRLAARNV PVGFGESWKKHLSGEFGKPY FIKLMGFVAE ERKHYTVYPP PHQVFTWTQM CDIKDVKVVILGQDPYHGPN QAHGLCFSVQ RPVPPPPSLE NIYKELSTDI EDFVHPGHGDLSGWAKQGVL LLNAVLTVRA HQANSHKERG WEQFTDAVVS WLNQNSNGLVFLLWGSYAQK KGSAIDRKRH HVLQTAHPSP LSVYRGFFGC RHFSKTNELLQKSGKKPIDW KEL
Applicable Applications for UNG recombinant protein
Positive Control; Immunogen; SDS-PAGE; Western Blot (WB).
Organism Species
Homo sapiens (Human)
Source
Prokaryotic expression
Residues
Met1~Leu313
Tags
N-terminal His Tag
Subcellular Location
Mitochondrion
Traits
Freeze-dried powder
Predicted isoelectric point
9.4
Usage
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
SDS-Page
SDS-Page
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted Molecular Mass: 38.3kDa
Accurate Molecular Mass: 40kDa as determined by SDS-PAGE reducing conditions.
Accurate Molecular Mass: 40kDa as determined by SDS-PAGE reducing conditions.
NCBI Official Full Name
uracil DNA glycosylase
NCBI Official Symbol
UNG
NCBI Official Synonym Symbols
ATUNG; UNG; uracil dna glycosylase
NCBI Protein Information
uracil DNA glycosylase
UniProt Protein Name
Uracil-DNA glycosylase, mitochondrial
Protein Family
UniProt Gene Name
UNG
UniProt Synonym Gene Names
UDG
UniProt Entry Name
UNG_ARATH
NCBI Description
Encodes a uracil-DNA glycosylase (UDG) involved in a base excision DNA repair pathway in mitochondria.
Uniprot Description
Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine. More active on U:G, U:T and U:C mispairs than on U:A pairs. Highly specific for uracil and no activity with 5-substituted uracil or cytosine derivatives. Required for initiation of base excision repair (BER) of uracil.
Research Articles on UNG
Similar Products
Product Notes
The UNG ung (Catalog #AAA2031790) is a Recombinant Protein produced from E.coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Uracil DNA Glycosylase can be used in a range of immunoassay formats including, but not limited to, Positive Control; Immunogen; SDS-PAGE; Western Blot (WB). Researchers should empirically determine the suitability of the UNG ung for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.MIGQKTLYS F FSPSPARKRH APSPEPAVQG TGVAGVPEES GDAAAIPAKK APAGQEEPGT PPSSPLSAEQ LDRIQRNKAA ALLRLAARNV PVGFGESWKK HLSGEFGKPY FIKLMGFVAE ERKHYTVYPP PHQVFTWTQM CDIKDVKVVI LGQDPYHGPN QAHGLCFSVQ RPVPPPPSLE NIYKELSTDI EDFVHPGHGD LSGWAKQGVL LLNAVLTVRA HQANSHKERG WEQFTDAVVS WLNQNSNGLV FLLWGSYAQK KGSAIDRKRH HVLQTAHPSP LSVYRGFFGC RHFSKTNELL QKSGKKPIDW KEL. It is sometimes possible for the material contained within the vial of "Uracil DNA Glycosylase, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.
Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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