MAT1A recombinant protein

MAT1A, 1-395aa, Human, His tag, E Coli

Cat. Num. AAA203716

• Gene Names: MAT1A; MAT; SAMS; MATA1; SAMS1
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: MAT1A; 1-395aa; Human; His tag; E Coli; S-adenosylmethionine synthetase; MAT; MATA1; SAMS; SAMS1; methionine adenosyltransferase I alpha; ; MAT1A recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 1mM DTT, 0.1M NaCl, 10% glycerol
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHS SGLVPRGSHM NGPVDGLCDH SLSEGVFMFT SESVGEGHPD KICDQISDAV LDAHLKQDPN AKVACETVCK TGMVLLCGEI TSMAMVDYQR VVRDTIKHIG YDDSAKGFDF KTCNVLVALE QQSPDIAQCV HLDRNEEDVG AGDQGLMFGY ATDETEECMP LTIILAHKLN ARMADLRRSG LLPWLRPDSK TQVTVQYMQD NGAVIPVRIH TIVISVQHNE DITLEEMRRA LKEQVIRAVV PAKYLDEDTV YHLQPSGRFV IGGPQGDAGV TGRKIIVDTY GGWGAHGGGA FSGKDYTKVD RSAAYAARWV AKSLVKAGLC RRVLVQVSYA IGVAEPLSIS IFTYGTSQKT ERELLDVVHK NFDLRPGVIV RDLDLKKPIY QKTACYGHFG RSEFPWEVPR KLVF
• Sequence Length: 1-395

• Applicable Applications for MAT1A recombinant protein: SDS-PAGE
• Tag Information: His-tag
• Domain: 1-395aa
• Preparation and Storage: Can be stored 2°C to 8°C for 1 week. For long term storage, aliquot and store at 20°C to 80°C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing conditions and visualized by coomassie blue stain)

Related Product Information for MAT1A recombinant protein

MAT1A catalyzes the formation of S-adenosyltransferase (AdoMet) for methionine catabolism in the liver. MAT1A expression also correlates with a differentiated phenotype, whereas liver cells expressing MAT2A present a dedifferentiated phenotype and lowered AdoMet synthesis. Likewise, NFkappaB and TNFalpha cause a switch from MAT1A to MAT2A expression in human hepatocellular carcinoma (HCC), which facilitates cancer cell growth. Recombinant human MAT1A protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for MAT1A recombinant protein

Metabolism

References

Ding W., et al. (2009) Hepatology. 49 (4):1277-86. ; Tomasi ML., et al. (2009) Gastroenterology. 136 (3):1025-36.;

NCBI and Uniprot Product Information

• NCBI GI #: 4557737
• NCBI GeneID: 4143
• NCBI Accession #: NP_000420
• NCBI GenBank Nucleotide #: NM_000429.2
• UniProt Accession #: Q00266; Q5QP09; D3DWD5
• Molecular Weight: 45.8 kDa (415aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: S-adenosylmethionine synthase isoform type-1
• NCBI Official Synonym Full Names: methionine adenosyltransferase 1A
• NCBI Official Symbol: MAT1A
• NCBI Official Synonym Symbols: MAT; SAMS; MATA1; SAMS1
• NCBI Protein Information: S-adenosylmethionine synthase isoform type-1; S-adenosylmethionine synthase
• UniProt Protein Name: S-adenosylmethionine synthase isoform type-1
• Protein Family: S-adenosylmethionine synthase
• UniProt Gene Name: MAT1A
• UniProt Synonym Gene Names: AMS1; MATA1; AdoMet synthase 1; MAT 1; MAT-I/III

NCBI Description

This gene catalyzes a two-step reaction that involves the transfer of the adenosyl moiety of ATP to methionine to form S-adenosylmethionine and tripolyphosphate, which is subsequently cleaved to PPi and Pi. S-adenosylmethionine is the source of methyl groups for most biological methylations. The encoded protein is found as a homotetramer (MAT I) or a homodimer (MAT III) whereas a third form, MAT II (gamma), is encoded by the MAT2A gene. Mutations in this gene are associated with methionine adenosyltransferase deficiency. [provided by RefSeq, Jul 2008]

Uniprot Description

Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The reaction comprises two steps that are both catalyzed by the same enzyme: formation of S-adenosylmethionine (AdoMet) and triphosphate, and subsequent hydrolysis of the triphosphate.