UCHL3 recombinant protein

UCHL3 recombinant protein

Cat. Num. AAA515247

• Gene Names: UCHL3; UCH-L3
• Applications: Western Blot
• Synonyms: UCHL3; UCHL3 recombinant protein; UCH-L3

• Host: E Coli
• Form/Format: 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
• Sequence Length: 972

• Applicable Applications for UCHL3 recombinant protein: Western Blot (WB)
• Type: Recombinant Fusion Protein
• Species: Human
• Tag Information: His tag
• Expression System: E.coli
• Source Note: Recombinant full length human UCHL3 was expressed in E. coli cells
• Preparation and Storage: Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

SDS-PAGE

Related Product Information for UCHL3 recombinant protein

Recombinant full length human UCHL3 was expressed in E. coli cells using an N-terminal His tag.

Scientific Background: UCHL3 or ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase) is a member of the deubiquitinating enzyme family that catalyze the removal of ubiquitin from polypeptides. The deubiquitinating enzyme family is divided into five classes, depending on the mechanism of catalysis. UCHL3 has been implicated in the etiology of neurodegeneration (1). UCHL3 may hydrolyze the ubiquitinyl-N-epsilon amide bond of ubiquitinated proteins to regenerate ubiquitin for another catalytic cycle and UCHL3 genes contain a conserved CpG island (2).

Product Categories/Family for UCHL3 recombinant protein

Ubiquitin Proteins; Signaling Proteins - Ubiquitin Proteins

References

1. Saigoh, K. et.al: Intragenic deletion in the gene encoding ubiquitin carboxy-terminal hydrolase in gad mice. Nature Genet. 23: 47-51, 1999.
2. Kurihara, L. J.et.al: Expression and functional analysis of Uch-L3 during mouse development. Molec. Cell. Biol. 20: 2498-2504, 2000.

NCBI and Uniprot Product Information

• NCBI GI #: 401709953
• NCBI GeneID: 7347
• NCBI Accession #: NP_005993.1
• NCBI GenBank Nucleotide #: NM_006002
• UniProt Accession #: P15374; Q5TBK8; Q6IBE9; B2R970
• Molecular Weight: ~27 kDa
• NCBI Official Full Name: Homo sapiens ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase) (UCHL3), transcript variant 2, mRNA
• NCBI Official Synonym Full Names: ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)
• NCBI Official Symbol: UCHL3
• NCBI Official Synonym Symbols: UCH-L3
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase isozyme L3; ubiquitin thiolesterase; ubiquitin thioesterase L3
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase isozyme L3
• Protein Family: Ubiquitin carboxyl-terminal hydrolase
• UniProt Gene Name: UCHL3
• UniProt Synonym Gene Names: UCH-L3
• UniProt Entry Name: UCHL3_HUMAN

NCBI Description

The protein encoded by this gene is a member of the deubiquitinating enzyme family. Members of this family are proteases that catalyze the removal of ubiquitin from polypeptides and are divided into five classes, depending on the mechanism of catalysis. This protein may hydrolyze the ubiquitinyl-N-epsilon amide bond of ubiquitinated proteins to regenerate ubiquitin for another catalytic cycle. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Aug 2012]

Uniprot Description

Function: Deubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. Thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. Has a 10-fold preference for Arg and Lys at position P3", and exhibits a preference towards 'Lys-48'-linked Ubiquitin chains. Deubiquitinates ENAC in apical compartments, thereby regulating apical membrane recycling. Indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. Required for stress-response retinal, skeletal muscle and germ cell maintenance. May be involved in working memory. Can hydrolyze UBB(+1), a mutated form of ubiquitin which is not effectively degraded by the proteasome and is associated with neurogenerative disorders. Ref.1 Ref.8 Ref.13 Ref.16 Ref.17

Catalytic activity: Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal). Ref.8 Ref.14 Ref.19

Enzyme regulation: Inhibited by monoubiquitin and diubiquitin. Ref.13

Subunit structure: Preferentially binds diubiquitin; the interaction does not hydrolyze diubiquitin but, in vitro, inhibits the hydrolyzing activity on other substrates.

Subcellular location: Cytoplasm.

Tissue specificity: Highly expressed in heart, skeletal muscle, and testis. Ref.8

Miscellaneous: Identified as a tumor-specific antigen in colon cancer.

Sequence similarities: Belongs to the peptidase C12 family.