Rabbit UCHL3 Polyclonal Antibody | anti-UCHL3 antibody

UCHL3 Rabbit pAb

Cat. Num. AAA4759048

• Gene Names: UCHL3; UCH-L3
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity purified
• Synonyms: UCHL3; Polyclonal Antibody; UCHL3 Rabbit pAb; ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase); UCH-L3; anti-UCHL3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: pH7.4 PBS, 0.05% NaN3, 40% Glycerol
• Concentration: 0.3mg/ml (varies by lot)

• Applicable Applications for anti-UCHL3 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:500-1:2000; IHC-P: 1:25-1:100
• Immunogen: Full length fusion protein
• Conjugation: Unconjugated
• Modification: Unmodified
• Preparation and Storage: Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Western Blot analysis of UCHL3 in 293T, K562, Jurkat and 231 cell in order using UCHL3 Rabbit pAb at dilution 1/300)

Immunohistochemistry (IHC)

(Immunohistochemistry of UCHL3 in paraffin-embedded Human breast cancer tissue using UCHL3 Rabbit pAb at dilution 1/25. Predicted cell location: Cytoplasm or Nucleus)

Related Product Information for anti-UCHL3 antibody

The protein encoded by this gene is a member of the deubiquitinating enzyme family. Members of this family are proteases that catalyze the removal of ubiquitin from polypeptides and are divided into five classes, depending on the mechanism of catalysis. This protein may hydrolyze the ubiquitinyl-N-epsilon amide bond of ubiquitinated proteins to regenerate ubiquitin for another catalytic cycle. Alternative splicing results in multiple transcript variants that encode different protein isoforms.

NCBI and Uniprot Product Information

• NCBI GI #: 401709950
• NCBI GeneID: 7347
• NCBI Accession #: NP_001257881.1
• NCBI GenBank Nucleotide #: NM_001270952.1
• UniProt Accession #: P15374; Q5TBK8; Q6IBE9; B2R970
• Molecular Weight: 26,183 Da
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase isozyme L3 isoform 1
• NCBI Official Synonym Full Names: ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)
• NCBI Official Symbol: UCHL3
• NCBI Official Synonym Symbols: UCH-L3
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase isozyme L3; ubiquitin thioesterase L3; ubiquitin thiolesterase
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase isozyme L3
• Protein Family: Ubiquitin carboxyl-terminal hydrolase
• UniProt Gene Name: UCHL3
• UniProt Synonym Gene Names: UCH-L3
• UniProt Entry Name: UCHL3_HUMAN

NCBI Description

The protein encoded by this gene is a member of the deubiquitinating enzyme family. Members of this family are proteases that catalyze the removal of ubiquitin from polypeptides and are divided into five classes, depending on the mechanism of catalysis. This protein may hydrolyze the ubiquitinyl-N-epsilon amide bond of ubiquitinated proteins to regenerate ubiquitin for another catalytic cycle. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Aug 2012]

Uniprot Description

UCHL3: Deubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. Thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. Has a 10-fold preference for Arg and Lys at position P3. Deubiquitinates ENAC in apical compartments, thereby regulating apical membrane recycling. Indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin- signaling and insulin-induced adipogenesis. Required for stress- response retinal, skeletal muscle and germ cell maintenance. May be involved in working memory. Can hydrolyze UBB(+1), a mutated form of ubiquitin which is not effectively degraded by the proteasome and is associated with neurogenerative disorders. Preferentially binds diubiquitin; the interaction does not hydrolyze diubiquitin but, in vitro, inhibits the hydrolyzing activity on other substrates. Highly expressed in heart, skeletal muscle, and testis. Inhibited by monoubiquitin and diubiquitin. Belongs to the peptidase C12 family.

Protein type: Protease; EC 3.4.19.12; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 13q22.2

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: peptidase activity; ubiquitin binding; protein binding; ubiquitin-specific protease activity

Biological Process: ubiquitin-dependent protein catabolic process; protein catabolic process