UBE2W recombinant protein

UBE2W Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121218

• Purity: >97% as determined by SDS-PAGE
• Synonyms: UBE2W; UBE2W Protein; Human; Recombinant (His Tag); Human UBE2W Protein (His Tag); UBC-16 Protein; UBC16 Protein; ubiquitin-conjugating enzyme E2W (putative); UBE2W recombinant protein

• Host: E Coli
• Purity/Purification: >97% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 100mM Arg.0.1% Brij35, pH8.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Cys151

• Species: Human
• Predicted N Terminal: Met
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human UBE2W (Q96B02-12)(Met 1-Cys 151) was expressed, with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for UBE2W recombinant protein

Background: Ubiquitin-conjugating enzymes, also known as UBE2W, E2 enzymes and more rarely as ubiquitin-carrier enzymes, perform the second step of protein ubiquitination. The modification of protein with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2W is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. It accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. It also catalyzes monoubiquitination and "Lys-11"-linked polyubiquitination. UBE2W is also considered to regulate FANCD2 monoubiquitination. UBE2W exhibits ubiquitin conjugating enzyme activity and catalyzes the monoubiquitination of PHD domain of Fanconi anemia complementation group L (FANCL). Over-expression of UBE2W in cells promotes the monoubiquitination of FANCD2 and down-regulated UBE2W markedly reduces the UV irradiation-induced but not MMC-induced FANCD2 monoubiquitination.

References

Zhang Y, et al. (2008) Generation of mouse UBE2W antibody and analysis of UBE2W expression in mouse tissues. Sheng Wu Gong Cheng Xue Bao. 24(4): 547-52.Scaglione KM, et al. (2011) Ube2w and ataxin-3 coordinately regulate the ubiquitin ligase CHIP. Mol Cell. 43(4): 599-612.Zhang Y, et al. (2011) UBE2W interacts with FANCL and regulates the monoubiquitination of Fanconi anemia protein FANCD2. Mol Cells. 31(2): 113-22.

NCBI and Uniprot Product Information

• UniProt Accession #: Q96B022
• Molecular Weight: The recombinant human UBE2W comprises 166 amino acids and has a predicted molecular mass of 19.2kDa.. It migrates as an approximately 18KDa band in SDS-PAGE under reducing conditions.
• Protein Family: Ubiquitin-conjugating enzyme