SDS-PAGE
UBE2R2 (UBC3B) recombinant protein
UBE2R2 (UBC3B) recombinant protein
Gene Names
UBE2R2; UBC3B; CDC34B; E2-CDC34B
Applications
Western Blot
Synonyms
UBE2R2 (UBC3B); UBE2R2 (UBC3B) recombinant protein; CDC34B; E2-CDC34B; UBC3B; UBE2R2(UBC3B) Protein
Host
E Coli
Form/Format
50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
Sequence Length
4457
Applicable Applications for UBE2R2 (UBC3B) recombinant protein
Western Blot (WB)
Type
Recombinant Fusion Protein
Species
Human
Tag Information
His tag
Expression System
E.coli
Source Note
Recombinant human UBE2R2 (UBC3B) (2-end) was expressed in E. coli cells
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
SDS-PAGE
SDS-PAGE
Related Product Information for UBE2R2 (UBC3B) recombinant protein
Recombinant human UBE2R2 (UBC3B) (2-end) was expressed in E. coli cells using an N-terminal His tag.
Scientific Background: UBE2R2 (UBC3B) or ubiquitin-conjugating enzyme E2R 2 encodes a protein similar to the E2 ubiquitin conjugating enzyme UBC3/CDC34. CK2-dependent phosphorylation of this ubiquitin-conjugating enzyme functions by regulating beta-TrCP substrate recognition and induces its interaction with beta-TrCP therby enhancing beta-catenin degradation. CK2-dependent phosphorylation of CDC34 and UBC3B functions by regulating BTRC substrate recognition (1). UBE2R2 complements a yeast cdc34 temperature-sensitive mutant. Deletion and site-directed mutagenesis demonstrated that CK2 phosphorylated UBE2R2 in the C-terminal domain at serine-233; mutagenesis of the corresponding serine residue at position 231 of human CDC34/UBC3 showed that this was the major CK2-phosphorylation site in this protein.
Scientific Background: UBE2R2 (UBC3B) or ubiquitin-conjugating enzyme E2R 2 encodes a protein similar to the E2 ubiquitin conjugating enzyme UBC3/CDC34. CK2-dependent phosphorylation of this ubiquitin-conjugating enzyme functions by regulating beta-TrCP substrate recognition and induces its interaction with beta-TrCP therby enhancing beta-catenin degradation. CK2-dependent phosphorylation of CDC34 and UBC3B functions by regulating BTRC substrate recognition (1). UBE2R2 complements a yeast cdc34 temperature-sensitive mutant. Deletion and site-directed mutagenesis demonstrated that CK2 phosphorylated UBE2R2 in the C-terminal domain at serine-233; mutagenesis of the corresponding serine residue at position 231 of human CDC34/UBC3 showed that this was the major CK2-phosphorylation site in this protein.
Product Categories/Family for UBE2R2 (UBC3B) recombinant protein
References
1. Semplici, F. et.al: CK2-dependent phosphorylation of the E2 ubiquitin conjugating enzyme UBC3B induces its interaction with beta-TrCP and enhances beta-catenin degradation. Oncogene 21: 3978-3987, 2002.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~32 kDa
NCBI Official Full Name
Homo sapiens ubiquitin-conjugating enzyme E2R 2 (UBE2R2), mRNA
NCBI Official Synonym Full Names
ubiquitin-conjugating enzyme E2R 2
NCBI Official Symbol
UBE2R2
NCBI Official Synonym Symbols
UBC3B; CDC34B; E2-CDC34B
NCBI Protein Information
ubiquitin-conjugating enzyme E2 R2; ubiquitin-protein ligase R2; ubiquitin carrier protein R2; ubiquitin-conjugating enzyme UBC3B; ubiquitin-conjugating enzyme E2-CDC34B
UniProt Protein Name
Ubiquitin-conjugating enzyme E2 R2
UniProt Gene Name
UBE2R2
UniProt Synonym Gene Names
CDC34B; UBC3B
UniProt Entry Name
UB2R2_HUMAN
NCBI Description
Protein kinase CK2 is a ubiquitous and pleiotropic Ser/Thr protein kinase involved in cell growth and transformation. This gene encodes a protein similar to the E2 ubiquitin conjugating enzyme UBC3/CDC34. Studies suggest that CK2-dependent phosphorylation of this ubiquitin-conjugating enzyme functions by regulating beta-TrCP substrate recognition and induces its interaction with beta-TrCP, enhancing beta-catenin degradation. [provided by RefSeq, Jul 2008]
Uniprot Description
Function: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes monoubiquitination and 'Lys-48'-linked polyubiquitination. May be involved in degradation of katenin. Ref.1 Ref.8
Catalytic activity: ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine. Ref.1
Pathway: Protein modification; protein ubiquitination.
Subunit structure: When phosphorylated, interacts with beta-TrCP (BTRC). Ref.1
Sequence similarities: Belongs to the ubiquitin-conjugating enzyme family.
Research Articles on UBE2R2 (UBC3B)
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Product Notes
The UBE2R2 (UBC3B) ube2r2 (Catalog #AAA515256) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His tag!!Expression System||E.coli!!Source Note||Recombinant human UBE2R2 (UBC3B) (2-end) was expressed in E. coli cells. AAA Biotech's UBE2R2 (UBC3B) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the UBE2R2 (UBC3B) ube2r2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UBE2R2 (UBC3B), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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