Rabbit anti-Human UBE2R2 Polyclonal Antibody | anti-UBE2R2 antibody

UBE2R2, ID (UBE2R2, CDC34B, UBC3B, Ubiquitin-conjugating enzyme E2 R2, Ubiquitin carrier protein R2, Ubiquitin-conjugating enzyme E2-CDC34B, Ubiquitin-protein ligase R2) (Azide free) (HRP)

Cat. Num. AAA6360787

• Gene Names: UBE2R2; UBC3B; CDC34B; E2-CDC34B
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: UBE2R2; Polyclonal Antibody; ID (UBE2R2; CDC34B; UBC3B; Ubiquitin-conjugating enzyme E2 R2; Ubiquitin carrier protein R2; Ubiquitin-conjugating enzyme E2-CDC34B; Ubiquitin-protein ligase R2) (Azide free) (HRP); Ubiquitin-protein ligase R2; anti-UBE2R2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-UBE2R2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: UBE2R2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 141-169 amino acids from the Central region of human UBE2R2.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(UBE2R2 Antibody (Center) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the UBE2R2 antibody detected the UBE2R2 protein (arrow).)

Related Product Information for anti-UBE2R2 antibody

Protein kinase CK2 is a ubiquitous and pleiotropic Ser/Thr protein kinase involved in cell growth and transformation. This gene encodes a protein similar to the E2 ubiquitin conjugating enzyme UBC3/CDC34. Studies suggest that CK2-dependent phosphorylation of this ubiquitin-conjugating enzyme functions by regulating beta-TrCP substrate recognition and induces its interaction with beta-TrCP, enhancing beta-catenin degradation.

Product Categories/Family for anti-UBE2R2 antibody

Antibodies; Ubiquitin Ligases

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Yoshida, T., et al. Int. J. Mol. Med. 25(4):649-656(2010). Oguri, M., et al. Am. J. Hypertens. 23(1):70-77(2010). Ferreira, M.A., et al. Nat. Genet. 40(9):1056-1058(2008). Humphray, S.J., et al. Nature 429(6990):369-374(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 22212943
• NCBI GeneID: 54926
• NCBI Accession #: NP_060281.2
• NCBI GenBank Nucleotide #: NM_017811.3
• UniProt Accession #: Q712K3; Q9NX64; D3DRL5
• Molecular Weight: 27,166 Da
• NCBI Official Full Name: ubiquitin-conjugating enzyme E2 R2
• NCBI Official Synonym Full Names: ubiquitin-conjugating enzyme E2R 2
• NCBI Official Symbol: UBE2R2
• NCBI Official Synonym Symbols: UBC3B; CDC34B; E2-CDC34B
• NCBI Protein Information: ubiquitin-conjugating enzyme E2 R2; ubiquitin carrier protein R2; ubiquitin-conjugating enzyme E2-CDC34B; ubiquitin-conjugating enzyme UBC3B; ubiquitin-protein ligase R2
• UniProt Protein Name: Ubiquitin-conjugating enzyme E2 R2
• Protein Family: Ubiquitin-conjugating enzyme
• UniProt Gene Name: UBE2R2
• UniProt Synonym Gene Names: CDC34B; UBC3B
• UniProt Entry Name: UB2R2_HUMAN

NCBI Description

Protein kinase CK2 is a ubiquitous and pleiotropic Ser/Thr protein kinase involved in cell growth and transformation. This gene encodes a protein similar to the E2 ubiquitin conjugating enzyme UBC3/CDC34. Studies suggest that CK2-dependent phosphorylation of this ubiquitin-conjugating enzyme functions by regulating beta-TrCP substrate recognition and induces its interaction with beta-TrCP, enhancing beta-catenin degradation. [provided by RefSeq, Jul 2008]

Uniprot Description

UBC3B: an E2 ubiquitin conjugating enzyme similar to UBC3/CDC34. Phosphorylation of UBC3B by CK2 induces its interaction with beta-TrCP, regulating beta-TrCP substrate recognition and enhancing beta-catenin degradation.

Protein type: Ubiquitin ligase; Ligase; Ubiquitin conjugating system; EC 6.3.2.19

Chromosomal Location of Human Ortholog: 9p13.3

Cellular Component: cytoplasm

Molecular Function: small conjugating protein ligase activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ATP binding; ligase activity

Biological Process: protein monoubiquitination; proteasomal ubiquitin-dependent protein catabolic process