Ubiquitin-Conjugating Enzyme E2H Recombinant Protein | UBE2H recombinant protein

Recombinant Human Ubiquitin-Conjugating Enzyme E2H

Cat. Num. AAA144964

• Gene Names: UBE2H; GID3; UBC8; UBCH; UBCH2; E2-20K
• Purity: Greater than 95.0% as determined by SDS-PAGE.
• Synonyms: Ubiquitin-Conjugating Enzyme E2H; Recombinant Human Ubiquitin-Conjugating Enzyme E2H; UBE2H Human; Ubiquitin-Conjugating Enzyme E2H Human Recombinant; Ubiquitin-conjugating enzyme E2 H; UbcH2; Ubiquitin carrier protein H; Ubiquitin-conjugating enzyme E2-20K; Ubiquitin-protein ligase H; UBE2H; GID3; UBC8; UBCH; UBE2H recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 95.0% as determined by SDS-PAGE.
• Form/Format: The UBE2H solution (1mg/ml) contains 20mM Tris-HCl buffer, pH8.0, 10% glycerol, 1mM DTT and 50mM NaCl.; Sterile filtered colorless solution.
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMSSPSPG KRRMDTDVVK LIESKHEVTI LGGLNEFVVK FYGPQGTPYE GGVWKVRVDL PDKYPFKSPS IGFMNKIFHP NIDEASGTVC LDVINQTWTA LYDLTNIFES FLPQLLAYPN PIDPLNGDAA AMYLHRPEEY KQKIKEYIQK YATEEALKEQ EEGTGDSSSE SSMSDFSEDE AQDMEL.
• Sequence Length: 113

• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Related Product Information for UBE2H recombinant protein

Description: UBE2H Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 206 amino acids (1-183) and having a molecular mass of 23.1kDa.UBE2H is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Introduction: Ubiquitin-conjugating enzyme E2 H (UBE2H) is a member of the ubiquitin-conjugating enzyme family. Protein modification with ubiquitin is a vital cellular apparatus for directing abnormal or short-lived proteins for degradation. Ubiquitination requires at least 3 classes of enzymes: ubiquitin-activating enzymes (E1s) ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). UBE2H receives ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. UBE2H protein sequence is 100% identical to the mouse homolog and 98% identical to the frog and zebrafish homologs.

Product Categories/Family for UBE2H recombinant protein

Enzymes; Ubiquitin Conjugating Enzyme

NCBI and Uniprot Product Information

• NCBI GI #: 321267500
• NCBI GeneID: 7328
• NCBI Accession #: NP_001189427.1
• NCBI GenBank Nucleotide #: NM_001202498.1
• UniProt Accession #: P62256; P37286; Q7Z6F4; A4D1L6; C9JY93
• Molecular Weight: 17,174 Da
• NCBI Official Full Name: ubiquitin-conjugating enzyme E2 H isoform 3
• NCBI Official Synonym Full Names: ubiquitin-conjugating enzyme E2H
• NCBI Official Symbol: UBE2H
• NCBI Official Synonym Symbols: GID3; UBC8; UBCH; UBCH2; E2-20K
• NCBI Protein Information: ubiquitin-conjugating enzyme E2 H; GID complex subunit 3, UBC8 homolog; ubiquitin carrier protein H; ubiquitin-conjugating enzyme E2-20K; ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast); ubiquitin-conjugating enzyme E2H (homologous to yeast UBC8); ubiquitin-protein ligase H
• UniProt Protein Name: Ubiquitin-conjugating enzyme E2 H
• Protein Family: Ubiquitin-conjugating enzyme
• UniProt Gene Name: UBE2H
• UniProt Entry Name: UBE2H_HUMAN

NCBI Description

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein sequence is 100% identical to the mouse homolog and 98% identical to the frog and zebrafish homologs. Three alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms. [provided by RefSeq, Feb 2011]

Uniprot Description

UBE2H: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 11'- and 'Lys-48'-linked polyubiquitination. Capable, in vitro, to ubiquitinate histone H2A. Belongs to the ubiquitin-conjugating enzyme family.

Protein type: Ubiquitin ligase; Ligase; EC 6.3.2.19; Mitochondrial; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 7q32

Cellular Component: cytoplasm

Molecular Function: protein binding; small conjugating protein ligase activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ATP binding; ligase activity

Biological Process: ubiquitin-dependent protein catabolic process