UBE2G1 recombinant protein

Recombinant Human UBE2G1 Protein

Cat. Num. AAA2545378

• Gene Names: UBE2G1; UBC7; E217K; UBE2G
• Purity: > 85 % as determined by SDS-PAGE
• Synonyms: UBE2G1; Recombinant Human UBE2G1 Protein; E217K; UBC7; UBE2G; UBE2G1 recombinant protein

• Host: E Coli
• Purity/Purification: > 85 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, 10% glycerol, pH 7.5
• Sequence Length: 170

• Application Notes: The recombinant human UBE2G1 consists of 172 amino acids and has a calculated molecular mass of 19.5 kDa as estimated in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Gly
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for UBE2G1 recombinant protein

Background: UBE2G1 is a member of the ubiquitin-conjugating E2 family whose members perform the second step in the ubiquitination reaction. Initially identified as the main process for protein degradation, ubiquitination is believed nowadays to be crucial for a wider range of cellular processes. The outcome of the ubiquitin-conjugation reaction, and thereby the fate of the substrate, is heavily dependent on the number of ubiquitin molecules attached and how these ubiquitin molecules are inter-connected. To deal with this complexity and to allow adequate ubiquitination in time and space, a highly sophisticated conjugation machinery has been developed. In a sequential manner, ubiquitin becomes activated by an ubiquitin-activating enzyme (E1), which then transfers the ubiquitin to a group of ubiquitin-conjugating enzymes (E2s). Next, ubiquitin-loaded E2s are interacting with ubiquitin protein ligases (E3s) and ubiquitin is conjugated to substrates on recruitment by the E3. These three key enzymes are operating in a hierarchical system, wherein two E1s and 35 E2s have been found and hundreds of E3s have been identified in humans. 

Description: A DNA sequence encoding the human UBE2G1 (P62253) (Met 1-Glu 170) was expressed and purified, with additional two amino acids (Gly & Pro) at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 13489085
• NCBI GeneID: 7326
• NCBI Accession #: NP_003333.1
• NCBI GenBank Nucleotide #: NM_003342.4
• UniProt Accession #: P62253; Q99462; B2R7P2; D3DTK0
• Molecular Weight: 19,509 Da
• NCBI Official Full Name: ubiquitin-conjugating enzyme E2 G1
• NCBI Official Synonym Full Names: ubiquitin conjugating enzyme E2 G1
• NCBI Official Symbol: UBE2G1
• NCBI Official Synonym Symbols: UBC7; E217K; UBE2G
• NCBI Protein Information: ubiquitin-conjugating enzyme E2 G1
• UniProt Protein Name: Ubiquitin-conjugating enzyme E2 G1
• Protein Family: Ubiquitin-conjugating enzyme
• UniProt Gene Name: UBE2G1
• UniProt Synonym Gene Names: UBE2G

NCBI Description

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family and catalyzes the covalent attachment of ubiquitin to other proteins. The protein may be involved in degradation of muscle-specific proteins. [provided by RefSeq, Jul 2008]

Uniprot Description

UBE2G1: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 48'-, as well as 'Lys-63'-linked polyubiquitination. May be involved in degradation of muscle-specific proteins. Mediates polyubiquitination of CYP3A4. Belongs to the ubiquitin-conjugating enzyme family.

Protein type: EC 6.3.2.19; Ligase; Ubiquitin conjugating system; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 17p13.2

Cellular Component: cytoplasm

Molecular Function: ubiquitin protein ligase binding; ubiquitin-protein ligase activity

Biological Process: cellular protein catabolic process; ubiquitin-dependent protein catabolic process