UbcH6 recombinant protein

UbcH6 (human), (recombinant) (His-tag)

Cat. Num. AAA566721

• Gene Names: UBE2E1; UBCH6
• Purity: >=95% (SDS-PAGE)
• Synonyms: UbcH6; UbcH6 (human); (recombinant) (His-tag); UbcH6 recombinant protein

• Host: E Coli
• Purity/Purification: >=95% (SDS-PAGE)
• Form/Format: Liquid. In PBS, pH7.2, containing 1mM DTT and 10% glycerol

• Preparation and Storage: For long term storage, store at -80 degree C; While not recommended, it appears that the enzyme is stable to multiple freeze/thaw cycles; Shipping: Shipped on Dry Ice

Related Product Information for UbcH6 recombinant protein

Ubiquitinylation of proteins constitutes an important cellular mechanism for targeting short-lived proteins for degradation by the 26S proteasome. Three classes of enzymes are involved in the conjugation of ubiquitin to proteins. E1, the ubiquitin activating enzyme, activates ubiquitin through the ATP-dependent formation of a high-energy thiol ester bond between the carboxyl terminus of ubiquitin and the active-site cysteine within E1. This E1-activated ubiquitin is transferred to a cysteine residue of an E2, or ubiquitin-conjugating enzyme (UbC). E2 enzymes, either by themselves or in conjunction with E3 enzymes (ubiquitin ligases), then transfer ubiquitin to target proteins forming stable isopeptide bonds resulting in multi-ubiquitin chain formation. It is the diverse combinations of E2-E3 complexes that are thought to define substrate specificity.

UbcH6 is a integral membrane protein localized to the endoplasmic reticulum (ER), and possibly the nuclear envelope. Sequence comparison reveals that UbcH6 is a member of an evolutionally conserved subfamily of E2s that includes UbcH5 and the S. cerevisiae derived Ubc4. However, UbcH6 appears to be unique among these E2s since it contains an amino-terminal extension of approximately 40 amino acids, the functional significance of which extension is presently unknown. Confusingly, UbcH6 it is not related to the S. cerevisiae derived Ubc6. Regardless of amino acid sequence homologies, it appears that UbcH6 may be structurally more similar to UbcH5a than UbcH5a is itself to either UbcH5b or UbcH5c. Despite these sequence similarities, UbcH6 does not efficiently substitute for UbcH5 or S. cerevisiae derived Ubc4 in E6-AP-dependent protein ubiquitinylation or many other HECT-domain mediated events.

UbcH6 may be involved in the degradation of unprocessed protein precursors that are not translocated through the rough ER, turnover of membrane proteins such as cytochrome P450 2E14, and the control of transcription factors.

Product Categories/Family for UbcH6 recombinant protein

Cellular Analysis; Stem Cells; Proteostasis; Protein Synthesis & Folding; Posttranslational Modifications; Ubiquitin & UBL Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 321117185
• NCBI GeneID: 7324
• NCBI Accession #: NP_001189405.1
• NCBI GenBank Nucleotide #: NM_001202476.1
• UniProt Accession #: P51965; B2RBX4; C9J8K2; K4DI90
• Molecular Weight: 19,499 Da
• NCBI Official Full Name: ubiquitin-conjugating enzyme E2 E1 isoform 3
• NCBI Official Synonym Full Names: ubiquitin-conjugating enzyme E2E 1
• NCBI Official Symbol: UBE2E1
• NCBI Official Synonym Symbols: UBCH6
• NCBI Protein Information: ubiquitin-conjugating enzyme E2 E1; ubiquitin carrier protein E1; ubiquitin-conjugating enzyme E2E 1 (UBC4/5 homolog, yeast); ubiquitin-conjugating enzyme E2E 1 (homologous to yeast UBC4/5); ubiquitin-protein ligase E1
• UniProt Protein Name: Ubiquitin-conjugating enzyme E2 E1
• UniProt Gene Name: UBE2E1
• UniProt Synonym Gene Names: UBCH6
• UniProt Entry Name: UB2E1_HUMAN

NCBI Description

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Three alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jan 2011]

Uniprot Description

UBE2E1: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes the covalent attachment of ISG15 to other proteins. Mediates the selective degradation of short-lived and abnormal proteins. In vitro also catalyzes 'Lys-48'-linked polyubiquitination. Belongs to the ubiquitin-conjugating enzyme family.

Protein type: Ubiquitin ligase; Ligase; EC 6.3.2.19; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 3p24.2

Cellular Component: nucleoplasm; cytosol; nucleus; ubiquitin ligase complex

Molecular Function: protein binding; ISG15 conjugating enzyme activity; ubiquitin-protein ligase activity; ATP binding; ligase activity

Biological Process: ubiquitin-dependent protein catabolic process; ISG15-protein conjugation; histone monoubiquitination; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; cytokine and chemokine mediated signaling pathway; mitotic cell cycle spindle assembly checkpoint; histone H2B ubiquitination; protein ubiquitination; mitotic cell cycle