Thioredoxin Reductase 2, SelCys523Cys Recombinant Protein | TXNRD3 recombinant protein

Thioredoxin Reductase 2, SelCys523Cys, Recombinant, Human (Thioredoxin Reductase 2, Mitochondrial, Selenoprotein Z, SelZ, TR-beta, Thioredoxin Reductase TR3, TXNRD2, KIAA1652, TRXR2)

Cat. Num. AAA637265

• Gene Names: TXNRD3; TGR; TR2; TRXR3
• Purity: Highly Purified; ~95% (SDS-PAGE)
• Synonyms: Thioredoxin Reductase 2; SelCys523Cys; Recombinant; Human (Thioredoxin Reductase 2; Mitochondrial; Selenoprotein Z; SelZ; TR-beta; Thioredoxin Reductase TR3; TXNRD2; KIAA1652; TRXR2); TXNRD3 recombinant protein

• Host: E Coli
• Purity/Purification: Highly Purified; ~95% (SDS-PAGE)
• Form/Format: Supplied as a lyophilized powder from 20mM HEPES. Reconstitute with sterile ddH2O.

• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Testing Data

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Related Product Information for TXNRD3 recombinant protein

The mammalian thioredoxin reductases (TrxRs) are a family of selenocysteine-containing pyridine nucleotide-disulfide oxido-reductases. All the mammalian TrxRs are homologous to glutathione reductase with respect to primary structure including the conserved redox catalytic site (-Cys-Val-Asn-Val-Gly-Cys-) but distinctively with a C-terminal extension containing a catalytically active penultimate selenocysteine (SeCys) residue in the conserved sequence(-Gly-Cys-SeCys-Gly). TrxR is homodimeric protein in which each monomer includes an FAD prosthetic group, a NADPH binding site and a redox catalytic site. Electrons are transferred from NADPH via FAD and the active-site disulfide to C-terminal SeCys-containing redox center, which then reduces the substrate like thioredoxin. The members of TrxR family are 55 - 58kD in molecular size and composed of three isoforms including cytosolic TrxR1, mitochondrial TrxR2, and TrxR3, known as Trx and GSSG reductase (TGR). TrxR plays a key role in protection of cells against oxidative stress and redox-regulatory mechanism of transcription factors and varioMyBioSource phenomena.

Recombinant protein corresponding to aa37-524 from human Thioredoxin reductase 2 with N-His tag, expressed in E.coli. (Q99757)

Product Categories/Family for TXNRD3 recombinant protein

Molecular Biology; MB-Enzymes; Thioredoxin Reductase

NCBI and Uniprot Product Information

• NCBI GI #: 291045268
• NCBI GeneID: 114112
• NCBI Accession #: NP_001166984.1
• NCBI GenBank Nucleotide #: NM_001173513.1
• UniProt Accession #: Q86VQ6; Q6PIS8; Q9NNW6; Q9P101
• Molecular Weight: ~55.4kD
• NCBI Official Full Name: thioredoxin reductase 3 isoform 2
• NCBI Official Synonym Full Names: thioredoxin reductase 3
• NCBI Official Symbol: TXNRD3
• NCBI Official Synonym Symbols: TGR; TR2; TRXR3
• NCBI Protein Information: thioredoxin reductase 3; thioredoxin reductase 2; thioredoxin reductase TR2; thioredoxin glutathione reductase; thioredoxin and glutathione reductase
• UniProt Protein Name: Thioredoxin reductase 3
• Protein Family: Thioredoxin reductase
• UniProt Gene Name: TXNRD3
• UniProt Synonym Gene Names: TGR; TRXR3
• UniProt Entry Name: TRXR3_HUMAN

NCBI Description

This gene encodes a member of the family of pyridine nucleotide oxidoreductases. This protein catalyzes the reduction of thioredoxin, and is implicated in the defense against oxidative stress. It contains a selenocysteine (Sec) residue (which is essential for catalytic activity), encoded by a UGA codon, at the penultimate C-terminal position. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]

Uniprot Description

TXNRD3: Displays thioredoxin reductase, glutaredoxin and glutathione reductase activities. Catalyzes disulfide bond isomerization. Promotes disulfide bond formation between GPX4 and various sperm proteins and may play a role in sperm maturation by promoting formation of sperm structural components. Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.

Protein type: EC 1.8.1.9; Oxidoreductase

Chromosomal Location of Human Ortholog: 3q21.3

Cellular Component: endoplasmic reticulum; nucleus

Molecular Function: thioredoxin-disulfide reductase activity; FAD binding; electron carrier activity; protein disulfide oxidoreductase activity

Biological Process: cell redox homeostasis; multicellular organismal development; spermatogenesis; cell differentiation