Rabbit anti-Human TXNRD3 Polyclonal Antibody | anti-TXNRD3 antibody

TXNRD3, ID (TXNRD3, TGR, TRXR3, Thioredoxin reductase 3, Thioredoxin and glutathione reductase, Thioredoxin reductase TR2) (AP)

Cat. Num. AAA6360409

• Gene Names: TXNRD3; TGR; TR2; TRXR3
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TXNRD3; Polyclonal Antibody; ID (TXNRD3; TGR; TRXR3; Thioredoxin reductase 3; Thioredoxin and glutathione reductase; Thioredoxin reductase TR2) (AP); Thioredoxin reductase TR2; anti-TXNRD3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-TXNRD3 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: TXNRD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 304-333 amino acids from the Central region of human TXNRD3.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(TXNRD3 Antibody (Center) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the TXNRD3 antibody detected the TXNRD3 protein (arrow).)

Related Product Information for anti-TXNRD3 antibody

This gene encodes a member of the family of pyridine nucleotide oxidoreductases. This protein catalyzes the reduction of thioredoxin, and is implicated in the defense against oxidative stress. It contains a selenocysteine (Sec) residue (which is essential for catalytic activity), encoded by a UGA codon, at the penultimate C-terminal position. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-TXNRD3 antibody

Antibodies; Enzymes; Reductase

References

Gerashchenko, M.V., et al. J. Biol. Chem. 285(7):4595-4602(2010). Starr, J.M., et al. Mech. Ageing Dev. 129(12):745-751(2008). Harris, S.E., et al. BMC Genet. 8, 43 (2007): Kryukov, G.V., et al. Science 300(5624):1439-1443(2003). Sun, Q.A., et al. Proc. Natl. Acad. Sci. U.S.A. 98(7):3673-3678(2001).

NCBI and Uniprot Product Information

• NCBI GI #: 291045268
• NCBI GeneID: 114112
• NCBI Accession #: NP_001166984.1
• NCBI GenBank Nucleotide #: NM_001173513.1
• UniProt Accession #: Q86VQ6; Q6PIS8; Q9NNW6; Q9P101
• Molecular Weight: 70,683 Da
• NCBI Official Full Name: thioredoxin reductase 3 isoform 2
• NCBI Official Synonym Full Names: thioredoxin reductase 3
• NCBI Official Symbol: TXNRD3
• NCBI Official Synonym Symbols: TGR; TR2; TRXR3
• NCBI Protein Information: thioredoxin reductase 3; thioredoxin reductase 2; thioredoxin reductase TR2; thioredoxin glutathione reductase; thioredoxin and glutathione reductase
• UniProt Protein Name: Thioredoxin reductase 3
• Protein Family: Thioredoxin reductase
• UniProt Gene Name: TXNRD3
• UniProt Synonym Gene Names: TGR; TRXR3
• UniProt Entry Name: TRXR3_HUMAN

NCBI Description

This gene encodes a member of the family of pyridine nucleotide oxidoreductases. This protein catalyzes the reduction of thioredoxin, and is implicated in the defense against oxidative stress. It contains a selenocysteine (Sec) residue (which is essential for catalytic activity), encoded by a UGA codon, at the penultimate C-terminal position. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]

Uniprot Description

TXNRD3: Displays thioredoxin reductase, glutaredoxin and glutathione reductase activities. Catalyzes disulfide bond isomerization. Promotes disulfide bond formation between GPX4 and various sperm proteins and may play a role in sperm maturation by promoting formation of sperm structural components. Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.

Protein type: EC 1.8.1.9; Oxidoreductase

Chromosomal Location of Human Ortholog: 3q21.3

Cellular Component: endoplasmic reticulum; nucleus

Molecular Function: thioredoxin-disulfide reductase activity; FAD binding; electron carrier activity; protein disulfide oxidoreductase activity

Biological Process: cell redox homeostasis; multicellular organismal development; spermatogenesis; cell differentiation