Thioredoxin (Trx) Recombinant Protein | Trx recombinant protein

Recombinant Thioredoxin (Trx)

Cat. Num. AAA2009893

• Gene Names: Txn1; ADF; Txn; Trx1; AW550880
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Thioredoxin (Trx); Recombinant Thioredoxin (Trx); Trx recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with a N-terminal his-tag, its sequence is listed below.; MGHHHHHHSGSEF-MVKLIESKEA FQEALAAAGD KLVVVDFSAT WCGPCKMIKP FFHSLCDKYS NVVFLEVDVD DCQDVAADCE VKCMPTFQFY KKGQKVGEFS GANKEKLEAS ITEYA
• Sequence Length: 105

• Applicable Applications for Trx recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: MET1~Ala105 (Accession # P10639) with a N-terminal his-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 6755911
• NCBI GeneID: 22166
• NCBI Accession #: NP_035790.1
• NCBI GenBank Nucleotide #: NM_011660.3
• UniProt Accession #: P10639; Q52KC4; Q9D8R0
• Molecular Weight: 13.2kDa
• NCBI Official Full Name: thioredoxin
• NCBI Official Synonym Full Names: thioredoxin 1
• NCBI Official Symbol: Txn1
• NCBI Official Synonym Symbols: ADF; Txn; Trx1; AW550880
• NCBI Protein Information: thioredoxin; trx; ATL-derived factor
• UniProt Protein Name: Thioredoxin
• Protein Family: Histone-lysine N-methyltransferase
• UniProt Gene Name: Txn
• UniProt Synonym Gene Names: Txn1; Trx; ADF
• UniProt Entry Name: THIO_MOUSE

Uniprot Description

TXN: Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity. Homodimer; disulfide-linked. Interacts with TXNIP through the redox-active site. Interacts with MAP3K5 and CASP3. In case of infection, interacts with S.typhimurium protein slrP. Interacts with APEX1; the interaction stimulates the FOS/JUN AP-1 DNA- binding activity in a redox-dependent manner. Up-regulated by ionizing radiation. Belongs to the thioredoxin family.

Protein type: Nuclear receptor co-regulator

Cellular Component: mitochondrion; cell soma; axon; cytoplasm; dendrite; extracellular region; nucleus; cytosol

Molecular Function: protein binding; enzyme binding; protein disulfide oxidoreductase activity; peptide disulfide oxidoreductase activity; oxidoreductase activity, acting on sulfur group of donors, disulfide as acceptor

Biological Process: response to radiation; sulfate assimilation; negative regulation of protein export from nucleus; regulation of transcription, DNA-dependent; transcription, DNA-dependent; protein folding; cell redox homeostasis; transport; regulation of protein import into nucleus, translocation; negative regulation of transcription from RNA polymerase II promoter; positive regulation of peptidyl-serine phosphorylation; glycerol ether metabolic process; positive regulation of DNA binding