SDS-PAGE
PTK9 recombinant protein
PTK9 recombinant protein
SDS-PAGE
SDS-PAGE
Scientific Background: PTK9 is an actin monomer-binding protein conserved from yeast to mammals which has been localized to cortical G-actin-rich structures that may be regulated by the small GTPase RAC1. PTK9 has tyrosine kinase activity similar to that of recombinant FGFR2, with optimal activity over pH 6.5 to 7.4 and a preference for manganese over magnesium as a divalent cofactor (1). PTK9 plays a central role in regulation of actin dynamics and cell motility in lymphoma cell homeostasis in vivo and represent as a bona fide lymphoma drug target (2).
2. Meacham. et.al: In vivo RNAi screening identifies regulators of actin dynamics as key determinants of lymphoma progression. Nature Genet. 41: 1133-1137, 2009.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes twinfilin, an actin monomer-binding protein conserved from yeast to mammals. Studies of the mouse counterpart suggest that this protein may be an actin monomer-binding protein, and its localization to cortical G-actin-rich structures may be regulated by the small GTPase RAC1. [provided by RefSeq, Jul 2008]
Uniprot Description
Function: Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles
By similarity.
Subunit structure: Interacts with G-actin; ADP-actin form and capping protein (CP). May also be able to interact with TWF2 and phosphoinositides, PI(4,5)P2. When bound to PI(4,5)P2, it is down-regulated
By similarity.
Subcellular location: Cytoplasm. Cytoplasm › cytoskeleton
By similarity. Note: Diffuse cytoplasmic localization with perinuclear and G-actin-rich cortical actin structures sublocalization. Also found at membrane ruffles and cell-cell contacts
By similarity.
Tissue specificity: Expressed at high levels in the colon, testis, ovary, prostate and lung. Expressed at lower levels in the brain, bladder and heart. Not detected in liver. Ref.1
Post-translational modification: Phosphorylated on serine and threonine residues. Ref.1
Sequence similarities: Belongs to the actin-binding proteins ADF family. Twinfilin subfamily.Contains 2 ADF-H domains.
Caution: Was originally (Ref.1) thought to have protein tyrosine kinase activity.
Sequence caution: The sequence AAH43148.2 differs from that shown. Reason: Erroneous initiation. The sequence AAH68548.1 differs from that shown. Reason: Erroneous initiation. The sequence BAD92539.1 differs from that shown. Reason: Erroneous initiation.
Research Articles on PTK9
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Product Notes
The PTK9 twf1 (Catalog #AAA515265) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is GST tag!!Expression System||Sf9 insect cells using baculovirus!!Source Note||Recombinant human PTK9 (2-end) was expressed by baculovirus in Sf9 insect cells. AAA Biotech's PTK9 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the PTK9 twf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PTK9, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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