PTK9 Recombinant Protein | TWF1 recombinant protein

PTK9 Protein, Human, Recombinant

Cat. Num. AAA8120987

• Gene Names: TWF1; A6; PTK9
• Purity: >94% as determined by SDS-PAGE
• Synonyms: PTK9; PTK9 Protein; Human; Recombinant; Human TWF1/PTK9/Twinfilin-1 Protein; A6 Protein; MGC23788 Protein; MGC41876 Protein; twinfilin actin binding protein 1; TWF1 recombinant protein

• Host: E Coli
• Purity/Purification: >94% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4, 10% glycerol. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Asp252

• Species: Human
• Predicted N Terminal: Met
• Tag: N-cleavage
• Protein Construction: A DNA sequence encoding the human TWF1 isoform 4 (Q12792-4)(Met 1-Asp 252) was expressed and purified, with additional two amino acids (Gly & Pro) at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for TWF1 recombinant protein

Background: Twinfilin-1, also known as Protein A6, Protein tyrosine kinase 9, TWF1 and PTK9, is a cytoplasm protein that belongs to the actin-binding proteins ADF family and Twinfilin subfamily. Twinfilin-1 (TWF1/PTK9) is a highly conserved actin monomer-binding protein that regulates cytoskeletal dynamics in organisms from yeast to mammals. In addition to the mammalian twinfilin-1, a second protein with approximately 65% sequence identity to twinfilin-1 exists in mouse and humans. TWF1/PTK9 is expressed at high levels in the colon, testis, ovary, prostate and lung. It is expressed at lower levels in the brain, bladder and heart. It is not detected in liver. TWF1/PTK9 is an actin-binding protein involved in motile and morphological processes. It inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. TWF1/PTK9 seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles.

References

Beeler JF, et al.,1994, Mol Cell Biol 14 (2): 982-8. Palmgren S, et al., 2002, J. Cell. Sci. 115 (Pt 5): 881-6. Vartiainen MK, et al.,2003, J. Biol. Chem. 278 (36): 34347-55. Hassel S, et al.,2004, Proteomics 4 (5): 1346-58. Moseley,J.B. et al., 2006, J Cell Sci. 119 (Pt 8):1547-57.

NCBI and Uniprot Product Information

• NCBI GI #: 334848198
• NCBI GeneID: 5756
• NCBI Accession #: NP_001229326.1
• NCBI GenBank Nucleotide #: NM_001242397.1
• UniProt Accession #: Q12792; Q59G07; Q5U0B1; Q6FHJ1; Q6FHL6; Q6NUK9; Q86XL6; Q8TCD3; A8K5A8; B3KXS6; B4DLX9
• Molecular Weight: 28,823 Da
• NCBI Official Full Name: twinfilin-1 isoform 1
• NCBI Official Synonym Full Names: twinfilin actin-binding protein 1
• NCBI Official Symbol: TWF1
• NCBI Official Synonym Symbols: A6; PTK9
• NCBI Protein Information: twinfilin-1; A6 protein tyrosine kinase; PTK9 protein tyrosine kinase 9; protein A6; twinfilin, actin-binding protein, homolog 1
• UniProt Protein Name: Twinfilin-1
• Protein Family: Twinfilin
• UniProt Gene Name: TWF1
• UniProt Synonym Gene Names: PTK9
• UniProt Entry Name: TWF1_HUMAN

NCBI Description

This gene encodes twinfilin, an actin monomer-binding protein conserved from yeast to mammals. Studies of the mouse counterpart suggest that this protein may be an actin monomer-binding protein, and its localization to cortical G-actin-rich structures may be regulated by the small GTPase RAC1. [provided by RefSeq, Jul 2008]

Uniprot Description

A6: Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles. Interacts with G-actin; ADP-actin form and capping protein (CP). May also be able to interact with TWF2 and phosphoinositides, PI(4,5)P2. When bound to PI(4,5)P2, it is down- regulated. Expressed at high levels in the colon, testis, ovary, prostate and lung. Expressed at lower levels in the brain, bladder and heart. Not detected in liver. Belongs to the actin-binding proteins ADF family. Twinfilin subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 12q12

Cellular Component: focal adhesion; myofibril; perinuclear region of cytoplasm; cytoplasm; intercellular junction; filopodium; actin cytoskeleton

Molecular Function: actin monomer binding; phosphatidylinositol-4,5-bisphosphate binding; protein-tyrosine kinase activity; ATP binding

Biological Process: negative regulation of actin filament polymerization; sequestering of actin monomers; regulation of actin phosphorylation