tRNA-splicing endonuclease subunit Sen2 (TSEN2) Recombinant Protein | TSEN2 recombinant protein

Recombinant Human tRNA-splicing endonuclease subunit Sen2 (TSEN2)

Cat. Num. AAA1395756

• Gene Names: TSEN2; SEN2; PCH2B; SEN2L
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: tRNA-splicing endonuclease subunit Sen2 (TSEN2); Recombinant Human tRNA-splicing endonuclease subunit Sen2 (TSEN2); TSEN2 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-465, Full length protein
• Sequence: MAEAVFHAPKRKRRVYETYESPLPIPFGQDHGPLKEFKIFRAEMINNNVIVRNAEDIEQLYGKGYFGKGILSRSRPSFTISDPKLVAKWKDMKTNMPIITSKRYQHSVEWAAELMRRQGQDESTVRRILKDYTKPLEHPPVKRNEEAQVHDKLNSGMVSNMEGTAGGERPSVVNGDSGKSGGVGDPREPLGCLQEGSGCHPTTESFEKSVREDASPLPHVCCCKQDALILQRGLHHEDGSQHIGLLHPGDRGPDHEYVLVEEAECAMSEREAAPNEELVQRNRLICRRNPYRIFEYLQLSLEEAFFLVYALGCLSIYYEKEPLTIVKLWKAFTVVQPTFRTTYMAYHYFRSKGWVPKVGLKYGTDLLLYRKGPPFYHASYSVIIELVDDHFEGSLRRPLSWKSLAALSRVSVNVSKELMLCYLIKPSTMTDKEMESPECMKRIKVQEVILSRWVSSRERSDQDDL
• Sequence Length: 465

• Species: Human
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 223972632
• NCBI GeneID: 80746
• NCBI Accession #: NP_001138864.1
• NCBI GenBank Nucleotide #: NM_001145392.1
• UniProt Accession #: Q8NCE0; Q8WTW7; Q9BPU7; B7Z6K1; C9IZI7; G5E9Q3
• Molecular Weight: 45,532 Da
• NCBI Official Full Name: tRNA-splicing endonuclease subunit Sen2 isoform 1
• NCBI Official Synonym Full Names: tRNA splicing endonuclease subunit 2
• NCBI Official Symbol: TSEN2
• NCBI Official Synonym Symbols: SEN2; PCH2B; SEN2L
• NCBI Protein Information: tRNA-splicing endonuclease subunit Sen2
• UniProt Protein Name: tRNA-splicing endonuclease subunit Sen2
• UniProt Gene Name: TSEN2
• UniProt Synonym Gene Names: SEN2; HsSen2

NCBI Description

This gene encodes one of the subunits of the tRNA splicing endonuclease. This endonuclease catalyzes the first step in RNA splicing which is the removal of introns. Mutations in this gene have been associated with pontocerebellar hypoplasia type 2. A pseudogene has been identified on chromosome 4. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Feb 2009]

Uniprot Description

Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. Isoform 1 probably carries the active site for 5'-splice site cleavage. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3'-end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3'-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events. Isoform 2 is responsible for processing a yet unknown RNA substrate. The complex containing isoform 2 is not able to cleave pre-tRNAs properly, although it retains endonucleolytic activity.