Transient Receptor Potential Cation Channel Subfamily V Recombinant Protein | TRPV1 recombinant protein

Recombinant Transient Receptor Potential Cation Channel Subfamily V, Member 1 (TRPV1)

Cat. Num. AAA2033040

• Gene Names: TRPV4; SMAL; VRL2; BCYM3; CMT2C; SPSMA; TRP12; VROAC; HMSN2C; OTRPC4; SSQTL1
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Transient Receptor Potential Cation Channel Subfamily V; Recombinant Transient Receptor Potential Cation Channel Subfamily V; Member 1 (TRPV1); VR1; OTRPC1; Vanilloid Receptor Subtype 1; Capsaicin receptor; Osm-9-like TRP channel 1; Vanilloid receptor 1; TRPV1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: MKKWSSTDLG AAADPLQKDT CPDPLDGDPN SRPPPAKPQL STAKSRTRLF GKGDSEEAFP VDCPHEEGEL DSCPTITVSP VITIQRPGDG PTGARLLSQD SVAASTEKTL RLYDRRSIFE AVAQNNCQDL ESLLLFLQKS KKHLTDNEFK DPE
• Sequence Length: 764

• Applicable Applications for TRPV1 recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 20.4kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Met1~Glu153
• Organism Species: Human
• Tag: two N-terminal Tags, His-tag and T7-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 294459977
• NCBI GeneID: 59341
• NCBI Accession #: NP_001170904.1
• NCBI GenBank Nucleotide #: NM_001177433.1
• Molecular Weight: 85,884 Da
• NCBI Official Full Name: transient receptor potential cation channel subfamily V member 4 isoform c
• NCBI Official Synonym Full Names: transient receptor potential cation channel subfamily V member 4
• NCBI Official Symbol: TRPV4
• NCBI Official Synonym Symbols: SMAL; VRL2; BCYM3; CMT2C; SPSMA; TRP12; VROAC; HMSN2C; OTRPC4; SSQTL1
• NCBI Protein Information: transient receptor potential cation channel subfamily V member 4
• UniProt Protein Name: Transient receptor potential cation channel subfamily V member 4
• Protein Family: Transient receptor potential cation channel subfamily
• UniProt Gene Name: TRPV4
• UniProt Synonym Gene Names: VRL2; VROAC; TrpV4; OTRPC4; TRP12; VRL-2; VR-OAC
• UniProt Entry Name: TRPV4_HUMAN

NCBI Description

This gene encodes a member of the OSM9-like transient receptor potential channel (OTRPC) subfamily in the transient receptor potential (TRP) superfamily of ion channels. The encoded protein is a Ca2+-permeable, nonselective cation channel that is thought to be involved in the regulation of systemic osmotic pressure. Mutations in this gene are the cause of spondylometaphyseal and metatropic dysplasia and hereditary motor and sensory neuropathy type IIC. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2010]

Uniprot Description

TRPV4: a non-selective calcium permeant cation channel probably involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by low pH, citrate and phorbol esters. Increase of intracellular Ca(2+) potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism. Promotes cell-cell junction formation in skin keratinocytes and plays an important role in the formation and/or maintenance of functional intercellular barriers. Acts as a regulator of intracellular Ca(2+) in synoviocytes. Plays an obligatory role as a molecular component in the nonselective cation channel activation induced by 4-alpha-phorbol 12,13-didecanoate and hypotonic stimulation in synoviocytes and also regulates production of IL-8. Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV4 sub-subfamily. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Channel, cation; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 12q24.1

Cellular Component: adherens junction; apical plasma membrane; cell surface; cilium; cortical actin cytoskeleton; cytoplasmic microtubule; cytoplasmic vesicle; cytosol; filopodium; focal adhesion; growth cone; integral to membrane; lamellipodium; plasma membrane

Molecular Function: actin binding; actin filament binding; alpha-tubulin binding; ATP binding; beta-tubulin binding; calcium channel activity; calmodulin binding; cation channel activity; microtubule binding; osmosensor activity; protein binding; protein kinase binding; protein kinase C binding; SH2 domain binding; stretch-activated, cation-selective, calcium channel activity

Biological Process: actin cytoskeleton reorganization; actin filament organization; calcium ion transport; cell volume homeostasis; cellular calcium ion homeostasis; cortical microtubule organization and biogenesis; elevation of cytosolic calcium ion concentration; hyperosmotic salinity response; intercellular junction assembly; microtubule polymerization; multicellular organismal water homeostasis; osmosensory signaling pathway; positive regulation of microtubule depolymerization; positive regulation of vascular permeability; regulation of response to osmotic stress; response to mechanical stimulus; transmembrane transport; vasopressin secretion

Disease: Brachyolmia Type 3; Digital Arthropathy-brachydactyly, Familial; Hereditary Motor And Sensory Neuropathy, Type Iic; Metatropic Dysplasia; Parastremmatic Dwarfism; Scapuloperoneal Spinal Muscular Atrophy; Sodium Serum Level Quantitative Trait Locus 1; Spinal Muscular Atrophy, Distal, Congenital Nonprogressive; Spondyloepiphyseal Dysplasia, Maroteaux Type; Spondylometaphyseal Dysplasia, Kozlowski Type