tRNA (guanine (37)-N1)-methyltransferase Recombinant Protein | TRM5 recombinant protein
Recombinant Saccharomyces cerevisiae tRNA (guanine (37)-N1)-methyltransferase
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Synonyms
tRNA (guanine (37)-N1)-methyltransferase; Recombinant Saccharomyces cerevisiae tRNA (guanine (37)-N1)-methyltransferase; TRM5 recombinant protein
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE.
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Sequence Positions
45-499, full length protein
Sequence
KIPMCAVKFPEPKNISVFSKNFKNCILRVPRIPHVVKLNSSKPKDELTSVQNKKLKTADGNNTPVTKGVLLHESIHSVEDAYGKLPEDALAFLKENSAEIVPHEYVLDYDFWKAEEILRAVLPEQFLEEVPTGFTITGHIAHLNLRTEFKPFDSLIGQVILDKNNKIECVVDKVSSIATQFRTFPMKVIAGKSDSLVVEQKESNCTFKFDFSKVYWNSRLHTEHERLVKQYFQPGQVVCDVFAGVGPFAVPAGKKDVIVLANDLNPESYKYLKENIALNKVAKTVKSFNMDGADFIRQSPQLLQQWIQDEEGGKITIPLPLKKRHRSQQHNDQQPPQPRTKELIIPSHISHYVMNLPDSAISFLGNFRGIFAAHTKGATDTIQMPWVHVHCFEKYPPGDQVTEDELHARVHARIIAALKVTADDLPLNAVSLHLVRKVAPTKPMYCASFQLPANV
Sequence Length
455
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
56,514 Da
NCBI Official Full Name
tRNA (guanine) methyltransferase
NCBI Official Symbol
TRM5
NCBI Protein Information
tRNA (guanine) methyltransferase
UniProt Protein Name
tRNA (guanine(37)-N1)-methyltransferase
UniProt Gene Name
TRM5
Uniprot Description
Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding. Postspliced cytoplasmic tRNAs are imported into the nucleus, where this first step seems to take place, after which they are reexported to the cytoplasm, where the yW sythesis is completed by cytoplasmic enzymes.
Research Articles on TRM5
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Product Notes
The TRM5 trm5 (Catalog #AAA1155342) is a Recombinant Protein produced from E Coli or Yeast or Baculovirus or Mammalian Cell and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 45-499, full length protein. The amino acid sequence is listed below: KIPMCAVKFP EPKNISVFSK NFKNCILRVP RIPHVVKLNS SKPKDELTSV QNKKLKTADG NNTPVTKGVL LHESIHSVED AYGKLPEDAL AFLKENSAEI VPHEYVLDYD FWKAEEILRA VLPEQFLEEV PTGFTITGHI AHLNLRTEFK PFDSLIGQVI LDKNNKIECV VDKVSSIATQ FRTFPMKVIA GKSDSLVVEQ KESNCTFKFD FSKVYWNSRL HTEHERLVKQ YFQPGQVVCD VFAGVGPFAV PAGKKDVIVL ANDLNPESYK YLKENIALNK VAKTVKSFNM DGADFIRQSP QLLQQWIQDE EGGKITIPLP LKKRHRSQQH NDQQPPQPRT KELIIPSHIS HYVMNLPDSA ISFLGNFRGI FAAHTKGATD TIQMPWVHVH CFEKYPPGDQ VTEDELHARV HARIIAALKV TADDLPLNAV SLHLVRKVAP TKPMYCASFQ LPANV. It is sometimes possible for the material contained within the vial of "tRNA (guanine (37)-N1)-methyltransferase, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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