Bovine fibronectin 1 ELISA Kit | FN1 elisa kit

Bovine Fibronectin, FN ELISA Kit

Cat. Num. AAA701901

• Gene Names: FN1; FN
• Reactivity: Bovine
• Synonyms: fibronectin 1; Bovine Fibronectin; FN ELISA Kit; CIG; DKFZp686F10164; DKFZp686H0342; DKFZp686I1370; DKFZp686O13149; ED-B; FINC; FN; FNZ; GFND; GFND2; LETS; MSF; OTTHUMP00000206762; OTTHUMP00000206767; cold-insoluble globulin; migration-stimulating fa; FN1 elisa kit

• Reactivity: Bovine
• Specificity: This assay has high sensitivity and excellent specificity for detection of Bovine FN1. No significant cross-reactivity or interference between Bovine FN1 and analogues was observed.
• Sequence Length: 2478

• Samples: Serum, plasma, cell culture supernates, tissue homogenates and Cell lysates
• Detection Range: 1.56 ug/ml-100 ug/ml
• Sensitivity: 0.39 ug/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Immunity
• Protein Biological Process 3: Acute phase
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FN1 elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to FN Standards or samples are then added to the appropriate microtiter plate wells with a HRP-conjugated antibody preparation specific for FN to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain FN, HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of FN in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 218512156
• NCBI GeneID: 280794
• UniProt Accession #: P07589
• Molecular Weight: 272,154 Da
• NCBI Official Full Name: Fibronectin
• NCBI Official Synonym Full Names: fibronectin 1
• NCBI Official Symbol: FN1
• NCBI Official Synonym Symbols: FN
• NCBI Protein Information: fibronectin; fibronectin ED-A- region; fibronectin variable region; embryo-specific fibronectin 1 transcript; cumulus cell-specific fibronectin 1 transcript
• UniProt Protein Name: Fibronectin
• Protein Family: Fibronectin
• UniProt Gene Name: FN1
• UniProt Synonym Gene Names: FN
• UniProt Entry Name: FINC_BOVIN

Uniprot Description

Function: Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts