Human tripartite motif-containing 35 ELISA Kit | TRIM35 elisa kit
Human Tripartite motif-containing protein 35, TRIM35 ELISA Kit
Gene Names
TRIM35; HLS5; MAIR
Reactivity
Human
Synonyms
tripartite motif-containing 35; Human Tripartite motif-containing protein 35; TRIM35 ELISA Kit; Human Tripartite motif-containing protein 35 (TRIM35) ELISA kit; HLS5; KIAA1098; MAIR; MGC17233; ; TRIM35 elisa kit
Reactivity
Human
Specificity
No significant cross-reactivity or interference between Human TRIM35 and analogues was observed.
Samples
Serum, Plasma, Tissue Homogenate, Feces and Urine
Assay Type
Sandwich
Detection Range
0.25 ng/ml - 8 ng/ml.
Sensitivity
0.1 ng/ml.
Intended Uses
This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated TRIM35 concentrations in Human serum, plasma and other body fluids. Using Purified Human TRIM35 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add TRIM35 and TRIM35 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TRIM35 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Intra-assay Precision
Intra-assay CV (%) is less than 15%.
Inter-assay Precision
Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
Preparation and Storage
Store all reagents at 2-8 degree C
Related Product Information for TRIM35 elisa kit
Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated TRIM35 concentrations in Human serum, plasma and other body fluids. Using Purified Human TRIM35 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add TRIM35 and TRIM35 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TRIM35 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
56,540 Da
NCBI Official Full Name
tripartite motif-containing protein 35
NCBI Official Synonym Full Names
tripartite motif containing 35
NCBI Official Symbol
TRIM35
NCBI Official Synonym Symbols
HLS5; MAIR
NCBI Protein Information
tripartite motif-containing protein 35; tripartite motif-containing 35; hemopoietic lineage switch protein 5
UniProt Protein Name
Tripartite motif-containing protein 35
Protein Family
UniProt Gene Name
TRIM35
UniProt Synonym Gene Names
HLS5; KIAA1098
UniProt Entry Name
TRI35_HUMAN
NCBI Description
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The function of this protein has not been identified. [provided by RefSeq, Jul 2008]
Uniprot Description
TRIM35: May play a role as a tumor suppressor. Implicated in the cell death mechanism. Belongs to the TRIM/RBCC family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Ubiquitin conjugating system
Chromosomal Location of Human Ortholog: 8p21.2
Cellular Component: nucleoplasm; cytoplasm; nucleus
Molecular Function: zinc ion binding
Biological Process: negative regulation of cell proliferation; apoptosis; positive regulation of apoptosis; innate immune response; negative regulation of mitotic cell cycle
Research Articles on TRIM35
Similar Products
Product Notes
The Human TRIM35 trim35 (Catalog #AAA9317166) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9317166 ELISA Kit recognizes Human TRIM35. It is sometimes possible for the material contained within the vial of "tripartite motif-containing 35, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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