Typical Testing Data/Standard Curve (for reference only)
Human Cav-1 (Caveolin-1) CLIA Kit | Cav-1 clia kit
Human Cav-1 (Caveolin-1) CLIA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Cav-1. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Cav-1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Cav-1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Cav-1. You can calculate the concentration of Cav-1 in the samples by comparing the RLU value of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
The scaffolding protein encoded by this gene is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. The gene is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 mitogen-activated kinase cascade. Caveolin 1 and caveolin 2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. Mutations in this gene have been associated with Berardinelli-Seip congenital lipodystrophy. Alternatively spliced transcripts encode alpha and beta isoforms of caveolin 1.[provided by RefSeq, Mar 2010]
Uniprot Description
Caveolin-1: May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Involved in the costimulatory signal essential for T-cell receptor (TCR)- mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3- dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway. Homooligomer. Interacts with GLIPR2, NOSTRIN, SNAP25 and syntaxin. Interacts with rotavirus A NSP4. Interacts (via the N- terminus) with DPP4; the interaction is direct. Interacts with CTNNB1, CDH1 and JUP. Interacts with BMX and BTK. Expressed in muscle and lung, less so in liver, brain and kidney. Belongs to the caveolin family. 2 isoforms of the human protein are produced by alternative initiation.
Protein type: Motility/polarity/chemotaxis; Nuclear receptor co-regulator; Adaptor/scaffold
Chromosomal Location of Human Ortholog: 7q31.1
Cellular Component: peroxisomal membrane; cell surface; mitochondrion; basolateral plasma membrane; integral to plasma membrane; lipid particle; cytosol; acrosomal membrane; lipid raft; membrane; early endosome membrane; perinuclear region of cytoplasm; apical plasma membrane; basal plasma membrane; intracellular; cytoplasmic vesicle; endosome; endoplasmic reticulum membrane; focal adhesion; protein complex; endoplasmic reticulum; caveola; cell cortex; cilium; Golgi membrane; plasma membrane
Molecular Function: protein binding, bridging; identical protein binding; protease activator activity; cholesterol binding; Rac GTPase binding; patched binding; protein kinase binding; ATPase binding; protein binding; enzyme binding; syntaxin binding; protein heterodimerization activity; protein complex scaffold; structural molecule activity; nitric-oxide synthase binding; receptor binding
Biological Process: mammary gland involution; response to glucocorticoid stimulus; negative regulation of epithelial cell differentiation; positive regulation of endocytosis; negative regulation of tyrosine phosphorylation of Stat5 protein; nitric oxide homeostasis; negative regulation of BMP signaling pathway; calcium ion homeostasis; sequestering of lipid; protein localization; regulation of the force of heart contraction by chemical signal; regulation of fatty acid metabolic process; negative regulation of protein binding; microtubule polymerization; inactivation of MAPK activity; maintenance of cellular protein localization; regulation of smooth muscle contraction; response to drug; skeletal muscle development; organ regeneration; positive regulation of toll-like receptor 3 signaling pathway; cytosolic calcium ion homeostasis; negative regulation of nitric-oxide synthase activity; cellular response to starvation; receptor mediated endocytosis of virus by host; membrane depolarization; negative regulation of neuron differentiation; cholesterol homeostasis; response to bacterium; response to estrogen stimulus; negative regulation of endothelial cell proliferation; T cell costimulation; negative regulation of protein ubiquitination; positive regulation of endothelial cell proliferation; regulation of nitric-oxide synthase activity; response to calcium ion; response to progesterone stimulus; leukocyte migration; negative regulation of JAK-STAT cascade; lactation; positive regulation of protein binding; negative regulation of smooth muscle cell proliferation; positive regulation of microtubule polymerization; vesicle organization and biogenesis; negative regulation of peptidyl-serine phosphorylation; negative regulation of pinocytosis; negative regulation of transcription from RNA polymerase II promoter; nitric oxide metabolic process; mammary gland development; calcium ion transport; response to gamma radiation; negative regulation of cytokine and chemokine mediated signaling pathway; angiogenesis; vasculogenesis; response to nutrient; protein homooligomerization; vasoconstriction; cholesterol transport; negative regulation of MAPKKK cascade; negative regulation of nitric oxide biosynthetic process; MAPKKK cascade; cholesterol efflux; positive regulation of metalloenzyme activity; positive regulation of peptidyl-serine phosphorylation; regulation of peptidase activity; cellular calcium ion homeostasis; triacylglycerol metabolic process; positive regulation of protein ubiquitination; regulation of blood coagulation; response to hypoxia; positive regulation of vasoconstriction; vascular endothelial growth factor receptor signaling pathway; blood coagulation
Disease: Lipodystrophy, Congenital Generalized, Type 3; Partial Lipodystrophy, Congenital Cataracts, And Neurodegeneration Syndrome; Pulmonary Hypertension, Primary, 3
Research Articles on Cav-1
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Product Notes
The Human Cav-1 cav1 (Catalog #AAA2531197) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2531197 CLIA Kit recognizes Human Cav-1. It is sometimes possible for the material contained within the vial of "Cav-1 (Caveolin-1), CLIA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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