Rabbit Histone Deacetylase 3 Polyclonal Antibody | anti-HDAC3 antibody

Rabbit anti Histone Deacetylase 3

Cat. Num. AAA225118

• Gene Names: HDAC3; HD3; RPD3; RPD3-2
• Reactivity: Human, Mouse, Rat (Expected from Sequence)
• Applications: Chromatin Immunoprecipitation, Immunoprecipitation, Immunofluorescence, Immunoprecipitation, Immunohistochemistry, Western Blot
• Purity: Purified whole serum by affinity chromatography.
• Synonyms: Histone Deacetylase 3; Polyclonal Antibody; Rabbit anti Histone Deacetylase 3; Histone Deacetylase 3 Antibody; anti-HDAC3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat (Expected from Sequence)
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified whole serum by affinity chromatography.
• Form/Format: Liquid in phosphate buffered saline with 0.02% sodium azide, 50% glycerol.
• Sequence Length: 428

• Applicable Applications for anti-HDAC3 antibody: Chromatin Immunoprecipitation (ChIP), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: ChIP: 1:20-1:100; IF: 1:50-1:200; IHC-Paraffin: 1:50-1:100; IP: 1:50-1:200; WB: 1:500-1:2000
• Immunogen: Recombinant human histone deacetylase 3
• Preperation: Antiserum to histone deacetylase 3 was raised by repeated immunization of rabbits with highly purified antigen.
• Preparation and Storage: Store at -20 degree C.; Storage in frost-free freezers is not recommended. This product should be stored undiluted.; Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch

Western Blot (WB)

(Western blot analysis of cell lysates using Rabbit anti histone deacetylase 3 antibody)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded mouse liver using Rabbit anti histone deacetylase 3 antibody at a dilution of 1/100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human lung cancer using Rabbit anti histone deacetylase 3 antibody at a dilution of 1/100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human colon carcinoma using Rabbit anti histone deacetylase 3 antibody at a dilution of 1/100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human stomach using Rabbit anti histone deacetylase 3 antibody at a dilution of 1/100)

Immunofluorescence (IF)

(Immunofluorescence analysis of MCF7 cells using Rabbit anti histone deacetylase 3 antibody. DAPI (blue) was used for nuclear staining)

Chromatin Immunoprecipitation (ChIP)

(Chromatin immunoprecipitation analysis extracts of 293T cells, using Rabbit anti histone deacetylase 3 antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. The histogram was constructed by the ratios of the immunoprecipitated DNA to the input)

Related Product Information for anti-HDAC3 antibody

Rabbit anti histone deacetylase 3 antibody recognizes histone deacetylase 3 also known as HDAC3. Histone deacetylases are chromatin-modifying enzymes that are involved in regulation of many aspects of cell biology including tissue differentiation, autophagy, apoptosis, migration, mitosis and angiogenesis. Class I HDACs, which include HDAC1, 2, 3 and 8 are ubiquitously expressed in all tissues and have predominantly nuclear localization.

NCBI and Uniprot Product Information

• NCBI GI #: 3334210
• NCBI GeneID: 8841
• UniProt Accession #: O15379; O43268; Q9UEI5; Q9UEV0; D3DQE1
• Molecular Weight: 49,111 Da
• NCBI Official Full Name: Histone deacetylase 3
• NCBI Official Synonym Full Names: histone deacetylase 3
• NCBI Official Symbol: HDAC3
• NCBI Official Synonym Symbols: HD3; RPD3; RPD3-2
• NCBI Protein Information: histone deacetylase 3
• UniProt Protein Name: Histone deacetylase 3
• Protein Family: Histone deacetylase
• UniProt Gene Name: HDAC3
• UniProt Synonym Gene Names: HD3

NCBI Description

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), and some other non-histone substrates. Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Participates in the BCL6 transcriptional repressor activity by deacetylating the H3 'Lys-27' (H3K27) on enhancer elements, antagonizing EP300 acetyltransferase activity and repressing proximal gene expression. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation (PubMed:21444723, PubMed:23911289). Contributes, together with XBP1 isoform 1, to the activation of NFE2L2-mediated HMOX1 transcription factor gene expression in a PI3K/mTORC2/Akt-dependent signaling pathway leading to endothelial cell (EC) survival under disturbed flow/oxidative stress (PubMed:25190803).