Chicken Thyrotropin Releasing Hormone ELISA Kit | TRH elisa kit

Chicken Thyrotropin Releasing Hormone ELISA Kit

Cat. Num. AAA44339

• Gene Names: TRH; TRF
• Reactivity: Chicken
• Synonyms: Thyrotropin Releasing Hormone; Chicken Thyrotropin Releasing Hormone ELISA Kit; TRH elisa kit

• Reactivity: Chicken
• Specificity: No significant cross-reactivity or interference between Chicken TRH and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
• Assay Type: Sandwich
• Detection Range: 0.625 ?IU/ml - 20 ?IU/ml.
• Sensitivity: 0.1 ?IU/ml.
• Intended Uses: ELISA is a simple and highly sensitive method of analysis that allows for simultaneous and rapid quantification of a large number of samples. The assay is based on the specific recognition of the target compound (analyte/antigen) by antibodies which bind to the compound. The antigen-antibody complex is detected and measured with the aid of an enzyme-labeled antibody or antigen. Upon addition of a non-colored reagent, the enzyme produces a color reaction where the color intensity is directly or inversely proportional to the concentration of the analyte in the sample. This quantitative Sandwich ELISA kit is in tended to determinate TRH concentrations in Chicken serum, plasma, tissue homogenates, feces, urine and body fluids, and it is only for research, not for drug, household, therapeutic applications
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TRH elisa kit

Introduction: ELISA is a simple and highly sensitive method of analysis that allows for simultaneous and rapid quantification of a large number of samples. The assay is based on the specific recognition of the target compound (analyte/antigen) by antibodies which bind to the compound. The antigen-antibody complex is detected and measured with the aid of an enzyme-labeled antibody or antigen. Upon addition of a non-colored reagent, the enzyme produces a color reaction where the color intensity is directly or inversely proportional to the concentration of the analyte in the sample. This quantitative Sandwich ELISA kit is in tended to determinate TRH concentrations in Chicken serum, plasma, tissue homogenates, feces, urine and body fluids, and it is only for research!

References

Blockade of neurokinin-1 receptors in the ventral respiratory column does not affect breathing but alters neurochemical release. Clarissa Muere, Suzanne Neumueller, Samantha Olesiak, Justin Miller, Matthew R. Hodges, Lawrence Pan, Hubert V. Forster (2015)

NCBI and Uniprot Product Information

• NCBI GI #: 6005920
• NCBI GeneID: 7200
• NCBI Accession #: NP_009048.1
• NCBI GenBank Nucleotide #: NM_007117.4
• UniProt Accession #: P20396; Q2TB83; B2R8R1
• Molecular Weight: 27,404 Da
• NCBI Official Full Name: thyrotropin releasing hormone preproprotein
• NCBI Official Synonym Full Names: thyrotropin-releasing hormone
• NCBI Official Symbol: TRH
• NCBI Official Synonym Symbols: TRF
• NCBI Protein Information: thyrotropin releasing hormone; protirelin; prothyroliberin; TSH-releasing factor; thyrotropin-releasing factor
• UniProt Protein Name: Pro-thyrotropin-releasing hormone
• Protein Family: Thyrotropin-releasing hormone
• UniProt Gene Name: TRH
• UniProt Synonym Gene Names: Pro-TRH; TRH; TRF
• UniProt Entry Name: TRH_HUMAN

NCBI Description

This gene encodes a member of the thyrotropin-releasing hormone family. Cleavage of the encoded proprotein releases mature thyrotropin-releasing hormone, which is a tripeptide hypothalamic regulatory hormone. The human proprotein contains six thyrotropin-releasing hormone tripeptides. Thyrotropin-releasing hormone is involved in the regulation and release of thyroid-stimulating hormone, as well as prolactin. Deficiency of this hormone has been associated with hypothalamic hypothyroidism. [provided by RefSeq, May 2013]

Uniprot Description

TRH: Functions as a regulator of the biosynthesis of TSH in the anterior pituitary gland and as a neurotransmitter/ neuromodulator in the central and peripheral nervous systems. May promote hair shaft elongation, prolonge the hair cycle growth phase (anagen) and antagonized its termination by TGFB2. May also increase proliferation and inhibited apoptosis of hair matrix keratinocytes. Belongs to the TRH family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 3q13.3-q21

Cellular Component: plasma membrane; extracellular region; nucleus; secretory granule

Molecular Function: neuropeptide hormone activity; thyrotropin-releasing hormone activity

Biological Process: hormone-mediated signaling; negative regulation of glutamate secretion; eating behavior; positive regulation of insulin secretion; histamine metabolic process; signal transduction; adult walking behavior; response to ethanol; cell-cell signaling; response to hypoxia; response to glucose stimulus; response to cold; response to corticosterone stimulus; positive regulation of gamma-aminobutyric acid secretion

Disease: Thyrotropin-releasing Hormone Deficiency