Rabbit anti-Human RPL11 Polyclonal Antibody | anti-RPL11 antibody

RPL11, NT (RPL11, 60S ribosomal protein L11, CLL-associated antigen KW-12) (AP)

Cat. Num. AAA6343336

• Gene Names: RPL11; L11; DBA7; GIG34
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: RPL11; Polyclonal Antibody; NT (RPL11; 60S ribosomal protein L11; CLL-associated antigen KW-12) (AP); CLL-associated antigen KW-12; anti-RPL11 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human RPL11.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-RPL11 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 1-30 in the N-terminal region of human RPL11. Species sequence homology: bovine, mouse, porcine, rat
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in Jurkat cell line lysates (35ug/lane) usingMBS645998. This demonstrates that MBS645998 detected the RPL11 protein (arrow).)

Testing Data

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Related Product Information for anti-RPL11 antibody

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L5P family of ribosomal proteins. It is located in the cytoplasm. The protein probably associates with the 5S rRNA. Alternative splice variants encoding different isoforms may exist, but they have not been fully characterized. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq].

Product Categories/Family for anti-RPL11 antibody

Antibodies; Ribosomal Proteins

References

1. Sun, X.X., et al. J. Biol. Chem. 285(33):25812-25821(2010). 2. Konno, Y., et al. Haematologica 95(8):1293-1299(2010). 3. Dai, M.S., et al. J. Biol. Chem. 285(17):12587-12594(2010). 4. Holzel, M., et al. J. Biol. Chem. 285(9):6364-6370(2010). 5. Quarello, P., et al. Haematologica 95(2):206-213(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 15431290
• NCBI GeneID: 6135
• NCBI Accession #: NP_000966.2
• NCBI GenBank Nucleotide #: NM_000975.3
• UniProt Accession #: P62913; P25121; P39026; Q8TDH2; Q9Y674
• Molecular Weight: 20,252 Da
• NCBI Official Full Name: 60S ribosomal protein L11 isoform 1
• NCBI Official Synonym Full Names: ribosomal protein L11
• NCBI Official Symbol: RPL11
• NCBI Official Synonym Symbols: L11; DBA7; GIG34
• NCBI Protein Information: 60S ribosomal protein L11; CLL-associated antigen KW-12; cell growth-inhibiting protein 34
• UniProt Protein Name: 60S ribosomal protein L11
• Protein Family: 50S ribosomal protein
• UniProt Gene Name: RPL11
• UniProt Entry Name: RL11_HUMAN

NCBI Description

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L5P family of ribosomal proteins. It is located in the cytoplasm. The protein probably associates with the 5S rRNA. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Dec 2010]

Uniprot Description

RPL11: Binds to 5S ribosomal RNA. Required for rRNA maturation and formation of the 60S ribosomal subunits. Promotes nucleolar location of PML. Defects in RPL11 are the cause of Diamond-Blackfan anemia type 7 (DBA7). DBA7 is a form of Diamond-Blackfan anemia, a congenital non-regenerative hypoplastic anemia that usually presents early in infancy. Diamond-Blackfan anemia is characterized by a moderate to severe macrocytic anemia, erythroblastopenia, and an increased risk of malignancy. 30 to 40% of Diamond-Blackfan anemia patients present with short stature and congenital anomalies, the most frequent being craniofacial (Pierre-Robin syndrome and cleft palate), thumb and urogenital anomalies. Belongs to the ribosomal protein L5P family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleolus; Motility/polarity/chemotaxis; Ribosomal; Translation

Chromosomal Location of Human Ortholog: 1p36.1-p35

Cellular Component: membrane; cytoplasm; nucleolus; cytosol

Molecular Function: rRNA binding; protein binding; structural constituent of ribosome; RNA binding

Biological Process: SRP-dependent cotranslational protein targeting to membrane; viral reproduction; translation; ribosomal large subunit biogenesis and assembly; translational termination; viral infectious cycle; cellular protein metabolic process; translational elongation; translational initiation; mRNA catabolic process, nonsense-mediated decay; viral transcription; gene expression; rRNA processing; protein targeting

Disease: Diamond-blackfan Anemia 7