TNF Receptor-Associated 1 Recombinant Protein | TRAP1 recombinant protein

TNF Receptor-Associated Protein 1 (TRAP1); Human Recombinant

Cat. Num. AAA8400684

• Gene Names: TRAP1; HSP75; HSP 75; HSP90L; TRAP-1
• Purity: >95% by SDS-PAGE
• Synonyms: TNF Receptor-Associated 1; TNF Receptor-Associated Protein 1 (TRAP1); Human Recombinant; HSP75; Mitochondrial HSP90; MtHSP90; TRAP1 recombinant protein

• Host: E Coli
• Specificity: Interacts with EXT2; EXT1 and Retinoblastoma protein
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid. In 50mM TRIS; pH7.5; containing 150mM sodium chloride; 1mM DTT; and 1mM EDTA
• Sequence Positions: aa60-704

• Source: Human
• Endotoxin Level: <50 EU/mg purified protein (LAL test)
• Tag: N terminus His-Tag
• Preparation and Storage: Store at -80 degree C; Centrifuge the vial prior to opening.; Shipping: Dry Ice; Shelf Life: Est.~12 months; Country of Origin: USA

ELISA (EIA)

SDS-Page

Western Blot (WB)

Related Product Information for TRAP1 recombinant protein

The Hsp90 family of heat shock proteins represents one of the most abundantly expressed and highly conserved families of cellular chaperones whose expression can be upregulated under conditions of cellular stress. It includes cytoplasmic (Hsp90-alpha/beta), ER (Grp94) and mitochondrial (TRAP1) localized members. Hsp90 is structurally composed of an N-terminal ATP binding domain, a medial substrate-binding region, and a C-terminal dimerization motif. Hsp90 dimers function in cooperation with cochaperones to stabilize a multitude of client proteins substrates. TRAP1 (aka Hsp75) was originally identified as a chaperone binding partner for retinoblastoma protein. TRAP1 was later found by immunofluorescence to be localized to the mitochondria. TRAP1 is significantly more active as an ATPase due in part to the lack of regulatory elements in the C-terminus, but retains the ability to dimerize. However, it does not form stable complexes with typical cochaperones of Hsp90 like p23 and HOP4. TRAP1 has been identified as a protective element for cell survival, and has been suggested as a target for anti-cancer therapeutics.

Product Categories/Family for TRAP1 recombinant protein

Web Categories; Proteins and Enzymes; Proteins and Enzymes (A-Z); Signal Transduction; Enzymes & Proteases

NCBI and Uniprot Product Information

• NCBI GI #: 67477458
• NCBI GeneID: 10131
• UniProt Accession #: Q12931; O43642; O75235; Q9UHL5; B4DR68; D3DUC8; F5H897
• Molecular Weight: 704
• NCBI Official Full Name: Heat shock protein 75 kDa, mitochondrial
• NCBI Official Synonym Full Names: TNF receptor-associated protein 1
• NCBI Official Symbol: TRAP1
• NCBI Official Synonym Symbols: HSP75; HSP 75; HSP90L; TRAP-1
• NCBI Protein Information: heat shock protein 75 kDa, mitochondrial; TNFR-associated protein 1; tumor necrosis factor type 1 receptor-associated protein
• UniProt Protein Name: Heat shock protein 75 kDa, mitochondrial
• Protein Family: TNF receptor-associated protein
• UniProt Gene Name: TRAP1
• UniProt Synonym Gene Names: HSP75; HSP 75; TRAP-1
• UniProt Entry Name: TRAP1_HUMAN

NCBI Description

This gene encodes a mitochondrial chaperone protein that is member of the heat shock protein 90 (HSP90) family. The encoded protein has ATPase activity and interacts with tumor necrosis factor type I. This protein may function in regulating cellular stress responses. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013]

Uniprot Description

HSP75: Chaperone that expresses an ATPase activity. Belongs to the heat shock protein 90 family.

Protein type: Chaperone; Heat shock protein

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: nucleoplasm; membrane; mitochondrion; mitochondrial matrix; mitochondrial inner membrane; mitochondrial intermembrane space; lipid particle

Molecular Function: protein binding; unfolded protein binding; protein kinase binding; tumor necrosis factor receptor binding; ATP binding

Biological Process: response to stress