Rabbit TRAP1 Polyclonal Antibody | anti-TRAP1 antibody

TRAP1 polyclonal antibody

Cat. Num. AAA565279

• Gene Names: TRAP1; HSP75; HSP 75; HSP90L; TRAP-1
• Reactivity: Human, Mouse, Rat, Bovine
• Applications: Western Blot
• Purity: Protein A affinity purified.
• Synonyms: TRAP1; Polyclonal Antibody; TRAP1 polyclonal antibody; HSP75; Mitochondrial HSP90; MtHSP90; TNF receptor-associated protein 1; anti-TRAP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Bovine
• Clonality: Polyclonal
• Specificity: Recognizes human, mouse, rat and bovine TRAP1. Detects a band of ~72kDa by Western blot.
• Purity/Purification: Protein A affinity purified.
• Form/Format: Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
• Sequence Length: 704

• Applicable Applications for anti-TRAP1 antibody: Western Blot (WB)
• Application Notes: Western Blot (1:1,000, colorimetric); Suggested dilutions/conditions may not be available for all applications.; Detects a band of ~72kDa by Western blot.
• Immunogen: Recombinant human TRAP1.
• Preparation and Storage: Long term storage: -20 degree C; Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of TRAP1: Lane 1: MWM, Lane 2: TRAP1 (human), (recombinant) (His-tag), Lane 3: HeLa (Heat Shocked), (Cell Lysate), Lane 4: PC12 (Heat Shocked), (Cell Lysate), Lane 5: 3T3 (Heat Shocked), (Cell Lysate), Lane 6: MDBK Cell Lysate.)

Related Product Information for anti-TRAP1 antibody

The Hsp90 family of heat shock proteins represents one of the most abundantly expressed and highly conserved families of cellular chaperones whose expression can be upregulated under conditions of cellular stress. It includes cytoplasmic (Hsp90-alpha/beta), ER (Grp94) and mitochondrial (TRAP1) localized members. Hsp90 is structurally composed of an N-terminal ATP binding domain, a medial substrate-binding region, and a C-terminal dimerization motif. Hsp90 dimers function in cooperation with cochaperones to stabilize a multitude of client protein substrates. TRAP1 (aka Hsp75) was originally identified as a chaperone binding partner for retinoblastoma protein. TRAP1 was later found by immunofluorescence to be localized to the mitochondria. TRAP1 is significantly more active as an ATPase due in part to the lack of regulatory elements in the C-terminus, but retains the ability to dimerize. However, it does not form stable complexes with typical cochaperones of Hsp90 like p23 and HOP4. TRAP1 has been identified as a protective element for cell survival, and has been suggested as a target for anti-cancer therapeutics.

Product Categories/Family for anti-TRAP1 antibody

Cell Signaling; Cell Organelles & Structures; Protein Synthesis & Folding

NCBI and Uniprot Product Information

• NCBI GI #: 67477458
• NCBI GeneID: 10131
• UniProt Accession #: Q12931; O43642; O75235; Q9UHL5; B4DR68; D3DUC8; F5H897
• Molecular Weight: 74,267 Da
• NCBI Official Full Name: Heat shock protein 75 kDa, mitochondrial
• NCBI Official Synonym Full Names: TNF receptor-associated protein 1
• NCBI Official Symbol: TRAP1
• NCBI Official Synonym Symbols: HSP75; HSP 75; HSP90L; TRAP-1
• NCBI Protein Information: heat shock protein 75 kDa, mitochondrial
• UniProt Protein Name: Heat shock protein 75 kDa, mitochondrial
• Protein Family: TNF receptor-associated protein
• UniProt Gene Name: TRAP1
• UniProt Synonym Gene Names: HSP75; HSP 75; TRAP-1
• UniProt Entry Name: TRAP1_HUMAN

NCBI Description

This gene encodes a mitochondrial chaperone protein that is member of the heat shock protein 90 (HSP90) family. The encoded protein has ATPase activity and interacts with tumor necrosis factor type I. This protein may function in regulating cellular stress responses. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013]

Uniprot Description

HSP75: Chaperone that expresses an ATPase activity. Belongs to the heat shock protein 90 family.

Protein type: Heat shock protein; Chaperone

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: lipid particle; membrane; mitochondrial inner membrane; mitochondrial intermembrane space; mitochondrial matrix; mitochondrion; nucleoplasm

Molecular Function: ATP binding; protein binding; protein kinase binding; tumor necrosis factor receptor binding; unfolded protein binding

Biological Process: response to stress; translational attenuation