Western Blot (WB)
(Biotinylated (1ug/ml) staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.)
Goat LIMP2/SCARB2 Polyclonal Antibody | anti-SCARB2 antibody
Goat anti-LIMP2/SCARB2, Biotinylated Antibody
Western Blot: Approx 80kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 54.3kDa according to NP_005497.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Fujita et al, Biochem Biophys Res Commun.1992 Apr 30;184(2): 604-11.; PMID: 1374238). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 1-3ug/ml.
Western Blot (WB)
(Biotinylated (1ug/ml) staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.)
Western Blot (WB)
(Biotinylated (1ug/ml) staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a type III glycoprotein that is located primarily in limiting membranes of lysosomes and endosomes. Earlier studies in mice and rat suggested that this protein may participate in membrane transportation and the reorganization of endosomal/lysosomal compartment. The protein deficiency in mice was reported to impair cell membrane transport processes and cause pelvic junction obstruction, deafness, and peripheral neuropathy. Further studies in human showed that this protein is a ubiquitously expressed protein and that it is involved in the pathogenesis of HFMD (hand, foot, and mouth disease) caused by enterovirus-71 and possibly by coxsackievirus A16. Mutations in this gene caused an autosomal recessive progressive myoclonic epilepsy-4 (EPM4), also known as action myoclonus-renal failure syndrome (AMRF). Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Feb 2011]
Uniprot Description
SCARB2: Acts as a lysosomal receptor for glucosylceramidase (GBA) targeting. Defects in SCARB2 are the cause of progressive myoclonic epilepsy type 4 with or without renal failure (EPM4). An autosomal recessive progressive myoclonic epilepsy associated with renal failure in some cases. Cognitive function is preserved. Myoclonus is a brief, involuntary twitching of a muscle or a group of muscles. Cognitive function is preserved. Genetic variants in SCARB2 can act as modifier of the phenotypic expression and severity of Gaucher disease. Belongs to the CD36 family.
Protein type: Membrane protein, integral; Membrane protein, multi-pass; Receptor, misc.
Chromosomal Location of Human Ortholog: 4q21.1
Cellular Component: focal adhesion; integral to membrane; lysosomal lumen; lysosomal membrane; membrane
Molecular Function: enzyme binding; protein binding; viral receptor activity
Biological Process: entry of virus into host cell; protein targeting to lysosome
Disease: Epilepsy, Progressive Myoclonic 4, With Or Without Renal Failure
Research Articles on SCARB2
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Product Notes
The SCARB2 scarb2 (Catalog #AAA423587) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-LIMP2/SCARB2, Biotinylated Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's LIMP2/SCARB2 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 4000. Western Blot: Approx 80kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 54.3kDa according to NP_005497.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Fujita et al, Biochem Biophys Res Commun.1992 Apr 30;184(2): 604-11.; PMID: 1374238). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the SCARB2 scarb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: NKANIQFGDN GTTIS. It is sometimes possible for the material contained within the vial of "LIMP2/SCARB2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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